Institute of Molecular Pathology, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic.
Proteomics. 2010 Dec;10(24):4501-11. doi: 10.1002/pmic.201000248. Epub 2010 Nov 9.
The facultative intracellular bacterium Francisella tularensis is the causal agent of the serious infectious disease tularemia. Despite the dynamic progress, which has been made in last few years, important questions regarding Francisella pathogenicity still remain to be answered. Generally, secreted proteins play an important role in pathogenicity of intracellular microbes. In this study, we investigated the protein composition of the culture filtrate proteins of highly virulent F. tularensis subsp. tularensis, strain SCHU S4 and attenuated F. tularensis subsp. holarctica, live vaccine strain using a comparative proteomic analysis. The majority of proteins identified in this study have been implicated in virulence mechanisms of other pathogens, and several have been categorized as having moonlighting properties; those that have more than one unrelated function. This profiling study of secreted proteins resulted in the unique detection of acid phosphatase (precursor) A (AcpA), β-lactamase, and hypothetical protein FTT0484 in the highly virulent strain SCHU S4 secretome. The release of AcpA may be of importance for F. tularensis subsp. tularensis virulence due to the recently described AcpA role in the F. tularensis escape from phagosomes.
兼性细胞内细菌土拉弗朗西斯菌是严重传染病土拉菌病的病原体。尽管近年来取得了动态进展，但关于弗朗西斯菌致病性的一些重要问题仍有待回答。一般来说，分泌蛋白在细胞内微生物的致病性中起着重要作用。在这项研究中，我们使用比较蛋白质组学分析研究了高毒力土拉弗朗西斯菌亚种。塔伦亚种 SCHU S4 株和减毒土拉弗朗西斯菌亚种。 holarctica，活疫苗株的培养滤液蛋白的蛋白组成。本研究中鉴定的大多数蛋白质都与其他病原体的毒力机制有关，并且有几种被归类为具有“月光蛋白”特性；那些具有多个不相关功能的蛋白质。分泌蛋白的这种分析 profiling 研究导致在高度毒力菌株 SCHU S4 分泌组中独特地检测到酸性磷酸酶（前体）A（AcpA）、β-内酰胺酶和假设蛋白 FTT0484。由于最近描述的 AcpA 在土拉弗朗西斯菌逃避吞噬体中的作用，AcpA 的释放可能对土拉弗朗西斯菌亚种。塔伦亚种的毒力很重要。
