School of Molecular and Microbial Biosciences, The University of Sydney, Sydney, Australia.
Proteomics Clin Appl. 2010 Feb;4(2):228-48. doi: 10.1002/prca.200900144. Epub 2010 Jan 14.
Technology development in the high throughput sciences of genomics, transcriptomics and proteomics, has been driven by bacteriological research. These organisms are excellent models for testing new methodology due to their comparatively small genome size, the relative ease of culturing large amounts of material, and the inherent biomedical, environmental and biotechnological interest in their underlying biology. Techniques developed in prokaryotes have since become applicable to higher organisms and human disease, opening vast research opportunities for understanding complex molecular processes. Pseudomonas aeruginosa is an excellent example of a microbe with fascinating properties suitable for stretching the boundaries of technology, and with underlying biology that remains poorly understood. P. aeruginosa is an opportunistic pathogen in humans and contains one of the largest genetic capabilities for a single-celled organism (approximately 5500 genes), which allows it to encode a wide variety of surface-associated and secreted virulence factors, as well as adapt to harsh environments, forming resistance to an array of antibacterial agents. While it is a major threat as a nosocomial pathogen, and particularly in the immunocompromised, it is also the most significant cause of mortality in patients suffering from the genetic disorder, cystic fibrosis. This review examines the role of proteomics in gaining a better understanding of the molecular basis of P. aeruginosa infection and persistence in the lungs of cystic fibrosis patients.
技术在高通量科学领域的发展，如基因组学、转录组学和蛋白质组学，一直受到细菌学研究的推动。由于其相对较小的基因组大小、大量培养物质的相对容易性，以及其潜在的生物医学、环境和生物技术对其基础生物学的兴趣，这些生物体是测试新方法的极好模型。在原核生物中开发的技术后来已适用于高等生物和人类疾病，为理解复杂的分子过程开辟了广阔的研究机会。铜绿假单胞菌是一种具有迷人特性的微生物的极好例子，适合扩展技术的边界，但其基础生物学仍未被充分理解。铜绿假单胞菌是人类中的机会性病原体，其遗传能力是单细胞生物中最大的之一（约 5500 个基因），这使其能够编码各种表面相关和分泌的毒力因子，并适应恶劣的环境，形成对多种抗菌药物的耐药性。虽然它作为医院获得性病原体是一个主要威胁，特别是在免疫功能低下的人群中，但它也是患有遗传性疾病囊性纤维化的患者死亡的最重要原因。这篇综述检查了蛋白质组学在更好地理解铜绿假单胞菌感染和在囊性纤维化患者肺部持续存在的分子基础方面的作用。
