Abteilung Proteinfunktion/Proteomics, Fachbereich Biologie, Technische Universität Kaiserslautern, Germany.
Proteomics Clin Appl. 2010 May;4(5):538-49. doi: 10.1002/prca.200900048. Epub 2010 Mar 2.
We want to identify proteins that are part of or associated with the plasma membrane of the human feto-maternal barrier, which is crucially important for nutrient, gas, and waste exchange between the mother and the fetus. All transfer processes occur through one specialized endothelial cell layer, the multinuclear syncytiotrophoblast (STB). Specifically, the apical plasma membrane of the STB interacts with the maternal blood and is the site of initial transport processes across the placenta.
We used a proteomic approach that employed the enrichment of apical STB membranes isolated from healthy placentae by ultracentrifugation and saccharose gradient centrifugation steps in combination with 1-D SDS-PAGE and ESI-MS analysis.
We identified 296 different proteins, 175 of which were integral and peripheral membrane proteins, partially containing 1-12 transmembrane domains or lipid anchors. One hundred and sixty-one proteins (54%) were allocated to the plasma membrane.
A high number of transporters, receptors, and proteins involved in signal transduction processes and vesicular trafficking were identified for the first time at the feto-maternal barrier. Our results are valuable sources for further studies of the cell physiology of the healthy placenta at the time of birth or the pathophysiology of several pregnancy disorders.
我们希望鉴定出与人胎母屏障的质膜相关或结合的蛋白质，该质膜对母体与胎儿之间的营养、气体和废物交换至关重要。所有的转运过程都通过一层特殊的内皮细胞层，即多核合胞滋养层（STB）进行。具体来说，STB 的顶质膜与母体血液相互作用，是胎盘内初始转运过程的发生部位。
我们采用了一种蛋白质组学方法，该方法通过超离心和蔗糖梯度离心步骤富集健康胎盘的顶质膜，结合 1-D SDS-PAGE 和 ESI-MS 分析。
我们鉴定出 296 种不同的蛋白质，其中 175 种是完整的和外周质膜蛋白，部分含有 1-12 个跨膜域或脂质锚。161 种蛋白质（54%）被分配到质膜中。
首次在胎母屏障处鉴定出大量转运体、受体和参与信号转导过程和囊泡运输的蛋白质。我们的结果为进一步研究出生时健康胎盘的细胞生理学或几种妊娠疾病的病理生理学提供了有价值的资源。
