Department of Physiology and Cell Biology, Ohio State University, 304 Hamilton Hall, 1645 Neil Ave., Columbus, OH 43210, USA.
Placenta. 2012 Mar;33(3):207-13. doi: 10.1016/j.placenta.2011.12.010. Epub 2012 Jan 4.
As a highly vascularized tissue, the placenta mediates gas and solute exchange between maternal and fetal circulations. In the human placenta, the interface with maternal blood is a unique epithelial structure known as the syncytiotrophoblast. Previously we developed a colloidal-silica based method to generate highly enriched preparations of the apical plasma membrane of the syncytiotrophoblast. Using similar preparations, a proteomics assessment of this important sub-proteome has identified 340 proteins as part of this apical membrane fraction. The expression of 38 of these proteins was previously unknown in the human placental syncytiotrophoblast. Together with previous studies, the current proteomic database expands our knowledge of the proteome of the syncytiotrophoblast apical plasma membrane from normal placentas to include more than 500 proteins. This database is a valuable resource for future comparisons to diseased placentas. Additionally, this data set provides a basis for further experimental studies of placenta and trophoblast function.
作为一种高度血管化的组织，胎盘在母体和胎儿循环之间介导气体和溶质交换。在人类胎盘，与母体血液的界面是一种独特的上皮结构，称为合体滋养层。以前，我们开发了一种基于胶体二氧化硅的方法来生成高度富集的合体滋养层顶膜制剂。使用类似的制剂，对这个重要的亚蛋白组进行蛋白质组学评估，鉴定出 340 种蛋白质作为这个顶膜部分的一部分。这些蛋白质中有 38 种以前在人类胎盘合体滋养层中是未知的。结合以前的研究，当前的蛋白质组数据库扩展了我们对正常胎盘合体滋养层顶膜蛋白质组的认识，包括 500 多种蛋白质。这个数据库是未来与患病胎盘进行比较的宝贵资源。此外，这个数据集为胎盘和滋养层功能的进一步实验研究提供了基础。
