Green Jon, Grout Brian
Parque Tecnologico de Vizcaya, Vizcaya, Spain.
Cryo Letters. 2010 Jul-Aug;31(4):341-6.
Direct cryopreservation of overwintering, dormant buds has been applied to nine blackcurrant cultivars, using a 7 day dehydration period at c.-20° C before plunging directly into liquid nitrogen. The buds on shoots thawed from -20° C showed normal development simply by standing them in water and all the cultivars could be successfully recovered (> 58%) by grafting. None of the shoots thawed from liquid nitrogen showed any development after standing in water and all of the grafts failed. Shoots thawed from liquid nitrogen showed significant damage to xylem transport, and the cortical tissues necessary fro successful grafting showed significant loss of membrane semipermeability. However, buds excised from shoots immediately after thawing from liquid nitrogen were viable and could be recovered using in vitro culture. Survival ranged from 88 to 55%, depending upon cultivar.
已将越冬休眠芽的直接冷冻保存应用于九个黑加仑品种，方法是在直接投入液氮之前，在约-20°C下进行7天的脱水处理。从-20°C解冻的枝条上的芽只需放在水中就能正常发育，并且通过嫁接所有品种都能成功恢复（>58%）。从液氮中解冻的枝条在水中放置后均未显示出任何发育迹象，所有嫁接均失败。从液氮中解冻的枝条显示木质部运输受到严重损害，成功嫁接所需的皮层组织显示出膜半透性的显著丧失。然而，从液氮中解冻后立即从枝条上切下的芽是有活力的，可以通过体外培养恢复。存活率在88%至55%之间，取决于品种。
