感染番茄丛矮病毒（TBSV）的番茄。

Tomato bushy stunt virus (TBSV) infecting Lycopersicon esculentum.

机构信息

Mubarak City for Scientific Research and Technology Application, New Burg Al-Arab, Alexandria, Egypt.

出版信息

Z Naturforsch C J Biosci. 2010 Sep-Oct;65(9-10):619-26. doi: 10.1515/znc-2010-9-1015.

DOI:10.1515/znc-2010-9-1015

Abstract

Tomato bushy stunt virus (TBSV) was detected in tomato crop (Lycopersicon esculentum) in Egypt with characteristic mosaic leaf deformation, stunting, and bushy growth symptoms. TBSV infection was confirmed serologically by ELISA and calculated incidence was 25.5%. Basic physicochemical properties of a purified TBSV Egh isolate were identical to known properties of tombusviruses of isometric 30-nm diameter particles, 41-kDa coat protein and the genome of approximately 4800 nt. This is the first TBSV isolate reported in Egypt. Cloning and partial sequencing of the isolate showed that it is more closely related to TBSV-P and TBSV-Ch than TBSV-Nf and TBSV-S strains of the virus. However, it is distinct from the above strains and could be a new strain of the virus which further confirms the genetic diversity of tombusviruses.

摘要

番茄丛矮病毒（TBSV）在埃及的番茄作物（Lycopersicon esculentum）中被检测到，其特征为花叶畸形、矮化和丛生生长症状。TBSV 的感染通过 ELISA 血清学方法得到确认，发病率为 25.5%。纯化的 Egh 分离株的基本理化性质与等轴对称 30nm 直径颗粒、41kDa 外壳蛋白和大约 4800nt 的基因组的已知属性相一致。这是埃及首次报道的 TBSV 分离株。该分离株的克隆和部分测序表明，它与 TBSV-P 和 TBSV-Ch 更为密切相关，而与 TBSV-Nf 和 TBSV-S 株系则不相关。然而，它与上述株系不同，可能是该病毒的一个新株系，进一步证实了 TBSV 的遗传多样性。