Scholthof K B, Scholthof H B, Jackson A O
Department of Plant Biology, University of California, Berkeley 94720, USA.
Virology. 1995 Aug 1;211(1):324-8. doi: 10.1006/viro.1995.1410.
Tombusviruses, of which tomato bushy stunt virus (TBSV) is the type member, spontaneously generate defective interfering RNAs (DIs) that are known to interfere with viral accumulation and symptom development. We show that co-infection with TBSV and DIs causes a dramatic reduction in accumulation of TBSV subgenomic RNAs and corresponding TBSV proteins with a less dramatic reduced accumulation of the genomic RNA and the replicase proteins. Associated with this differentially regulated suppression was a greatly reduced expression of both the p19 protein, which is responsible for severe symptoms, and the p22 protein, which is associated with cell-to-cell movement of the virus. Therefore, the results suggest that the protective effect of DIs may be due to selective inhibition of p19 and p22 expression in addition to reduced replication of genomic RNA.
番茄丛矮病毒属，其中番茄丛矮病毒（TBSV）是典型成员，能自发产生缺陷干扰RNA（DIs），已知其会干扰病毒积累和症状发展。我们发现，TBSV与DIs共同感染会导致TBSV亚基因组RNA和相应TBSV蛋白的积累显著减少，而基因组RNA和复制酶蛋白的积累减少幅度较小。与这种差异调节的抑制作用相关的是，负责严重症状的p19蛋白和与病毒细胞间移动相关的p22蛋白的表达均大幅降低。因此，结果表明，DIs的保护作用可能不仅归因于基因组RNA复制减少，还在于对p19和p22表达的选择性抑制。
