Graduate School of Science and Engineering for Research, University of Toyama, 3190, Gofuku, Toyama 930-8555, Japan.
Anal Chem. 2011 Jan 1;83(1):182-92. doi: 10.1021/ac102195x. Epub 2010 Dec 7.
It is probably impossible to prepare high-performance liquid chromatography (HPLC) columns that have a completely homogeneous packing structure. Many reports in the literature show that the radial distributions of the mobile phase flow velocity and the local column efficiency are not flat, even in columns considered as good. A degree of radial heterogeneity seems to be a common property of all HPLC columns and an important source of peak tailing, which prevents the derivation of accurate information on chromatographic behavior from a straightforward analysis of elution peak profiles. This work reports on a numerical method developed to derive from recorded peak profiles the column efficiency at the column center, the degree of column radial heterogeneity, and the polynomial function that best represents the radial distributions of the flow velocity and the column efficiency. This numerical method was applied to two concrete examples of tailing peak profiles previously described. It was demonstrated that this numerical method is effective to estimate important parameters characterizing the radial heterogeneity of chromatographic columns.
制备具有完全均匀填充结构的高效液相色谱 (HPLC) 柱可能是不可能的。许多文献报道表明，即使在被认为是良好的柱子中，流动相流速和局部柱效率的径向分布也不平坦。径向非均质性似乎是所有 HPLC 柱的共同特性，也是峰拖尾的重要原因，这阻止了从洗脱峰轮廓的直接分析中得出有关色谱行为的准确信息。这项工作报道了一种数值方法，该方法旨在从记录的峰轮廓中推导出柱中心的柱效率、柱径向非均质性的程度以及最佳代表流速和柱效率径向分布的多项式函数。该数值方法应用于以前描述的两个拖尾峰轮廓的具体示例。结果表明，该数值方法可有效地估计表征色谱柱径向非均质性的重要参数。
