Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Hong Kong SAR, China.
Anal Chem. 2011 Jan 1;83(1):289-96. doi: 10.1021/ac102393f. Epub 2010 Dec 7.
Gaseous biogenic amines such as putrescine, spermidine, aniline, and trimethylamine are important biomolecules that play many crucial roles in metabolism and medical diagnostics. A chemodosimetric detection assay has been developed for those gaseous amines by Ru(II)-Eu(III) heterobimetallic complexes, K{Ru(II)((t)Bubpy)(CN)(4)Eu(III)(H(2)O)(4)} (where (t)Bubpy = 4,4'-di-tert-butyl-2,2'-bipyridine). Synthesis, X-ray crystal characterization, and spectroscopic properties of this Ru(II)-Eu(III) heterobimetallic complex were reported. Binding properties of the Ru(II)-Eu(III) complex with common gases revealed that this complex is very selective to gaseous amine molecules. Sensitivity of this complex toward the amines was found as ∼log k() = 4.5-4.8. Real time monitoring of gaseous biogenic amines was applied to real fish samples (Atlantic mackerel) by studying the spectrofluorimetric responses of the Ru(II)-Eu(III) complex toward different biogenic amine concentration. GC/MS studies were also used as a reference for the studies. A linear spectrofluorimetric response was found toward biogenic amine concentration in real fish samples. This complex was found to respond specifically to those biogenic amines down to 10 ppb.
气态生物胺，如腐胺、亚精胺、苯胺和三甲胺，是在代谢和医学诊断中发挥许多关键作用的重要生物分子。本文报道了一种通过 Ru(II)-Eu(III) 杂金属配合物，K{Ru(II)((t)Bubpy)(CN)(4)Eu(III)(H(2)O)(4)}（其中(t)Bubpy = 4,4'-二-叔丁基-2,2'-联吡啶），对这些气态胺进行化学计量检测的方法。该 Ru(II)-Eu(III) 杂金属配合物的合成、X 射线晶体结构表征和光谱性质进行了研究。该 Ru(II)-Eu(III) 配合物与常见气体的结合特性表明，该配合物对气态胺分子具有很高的选择性。该配合物对胺的灵敏度为∼log k() = 4.5-4.8。通过研究 Ru(II)-Eu(III) 配合物对不同生物胺浓度的光谱荧光响应，将其应用于真实的鱼样（大西洋鲭鱼）中对气态生物胺进行实时监测。GC/MS 研究也被用作参考。在真实的鱼样中，对生物胺浓度表现出线性的光谱荧光响应。该配合物被发现对这些生物胺具有特异性响应，最低检测浓度可达 10 ppb。
