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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Gildberg A, Olsen R L, Bjarnason J B

Institute of Fisheries Technology Research, Tromsø, Norway.

Comp Biochem Physiol B. 1990;96(2):323-30. doi: 10.1016/0305-0491(90)90382-4.

Three pepsins were purified from the gastric mucosa of Atlantic cod (Gadus morhua). 2. The enzymes, called Pepsin I and Pepsin IIa and b, had isoelectric points 6.9, 4.0 and 4.1, respectively, and digested hemoglobin at a maximal rate at a pH of approximately 3. 3. They resembled bovine cathepsin D in being unable to digest the mammalian pepsin substrate N-acetyl-L-phenylalanyl-3,5-diiodo-L-tyrosine. 4. Specificity constants (kcat/Km) for the cod pepsins were lower than for porcine pepsin, and they expressed higher substrate affinity and physiological efficiency at pH 3.5 than at pH 2. 5. The cod pepsins are glycoproteins, and their amino acid composition resembles that of porcine cathepsin D more than that of porcine pepsin. 6. The N-terminal sequence of Atlantic cod pepsins is substantially different from that of porcine pepsin. This indicates a significant evolutionary gap between fish and mammalian pepsins.

从大西洋鳕鱼（Gadus morhua）的胃黏膜中纯化出了三种胃蛋白酶。2. 这些酶分别称为胃蛋白酶I、胃蛋白酶IIa和IIb，其等电点分别为6.9、4.0和4.1，在pH约为3时以最大速率消化血红蛋白。3. 它们与牛组织蛋白酶D相似，无法消化哺乳动物胃蛋白酶底物N-乙酰-L-苯丙氨酰-3,5-二碘-L-酪氨酸。4. 鳕鱼胃蛋白酶的特异性常数（kcat/Km）低于猪胃蛋白酶，并且在pH 3.5时比在pH 2时表现出更高的底物亲和力和生理效率。5. 鳕鱼胃蛋白酶是糖蛋白，其氨基酸组成与猪组织蛋白酶D的相似性高于与猪胃蛋白酶的相似性。6. 大西洋鳕鱼胃蛋白酶的N端序列与猪胃蛋白酶的有很大不同。这表明鱼类和哺乳动物胃蛋白酶之间存在显著的进化差距。

Gildberg A, Olsen R L, Bjarnason J B

Institute of Fisheries Technology Research, Tromsø, Norway.

Comp Biochem Physiol B. 1990;96(2):323-30. doi: 10.1016/0305-0491(90)90382-4.

Three pepsins were purified from the gastric mucosa of Atlantic cod (Gadus morhua). 2. The enzymes, called Pepsin I and Pepsin IIa and b, had isoelectric points 6.9, 4.0 and 4.1, respectively, and digested hemoglobin at a maximal rate at a pH of approximately 3. 3. They resembled bovine cathepsin D in being unable to digest the mammalian pepsin substrate N-acetyl-L-phenylalanyl-3,5-diiodo-L-tyrosine. 4. Specificity constants (kcat/Km) for the cod pepsins were lower than for porcine pepsin, and they expressed higher substrate affinity and physiological efficiency at pH 3.5 than at pH 2. 5. The cod pepsins are glycoproteins, and their amino acid composition resembles that of porcine cathepsin D more than that of porcine pepsin. 6. The N-terminal sequence of Atlantic cod pepsins is substantially different from that of porcine pepsin. This indicates a significant evolutionary gap between fish and mammalian pepsins.

从大西洋鳕鱼（Gadus morhua）的胃黏膜中纯化出了三种胃蛋白酶。2. 这些酶分别称为胃蛋白酶I、胃蛋白酶IIa和IIb，其等电点分别为6.9、4.0和4.1，在pH约为3时以最大速率消化血红蛋白。3. 它们与牛组织蛋白酶D相似，无法消化哺乳动物胃蛋白酶底物N-乙酰-L-苯丙氨酰-3,5-二碘-L-酪氨酸。4. 鳕鱼胃蛋白酶的特异性常数（kcat/Km）低于猪胃蛋白酶，并且在pH 3.5时比在pH 2时表现出更高的底物亲和力和生理效率。5. 鳕鱼胃蛋白酶是糖蛋白，其氨基酸组成与猪组织蛋白酶D的相似性高于与猪胃蛋白酶的相似性。6. 大西洋鳕鱼胃蛋白酶的N端序列与猪胃蛋白酶的有很大不同。这表明鱼类和哺乳动物胃蛋白酶之间存在显著的进化差距。