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铜对食用蛙肝脏色素细胞酪氨酸酶的影响

Effects of copper on the tyrosinase of liver pigment cells from Rana esculenta L.

作者信息

Cicero R, Gallone A, Maida I, Pintucci G

机构信息

Istituto di Biologia Generale, Facoltà di Medicina e Chirurgia dell'Università di Bari, Italy.

出版信息

Comp Biochem Physiol B. 1990;96(2):393-7. doi: 10.1016/0305-0491(90)90394-9.

DOI:10.1016/0305-0491(90)90394-9
PMID:2113847
Abstract
  1. The liver pigment cells of R. esculenta L. constitute a peculiar pigment cell system of histiocytic nature and contain a tyrosinase-like activity localized in the protein component of melanosomes. 2. The effects of addition and/or removal of Cu on the DOPA-oxidase activity of the system were studied. 3. It was concluded that: (a) this tyrosinase behaves as a Cu-enzyme; (b) Cu could be involved in the regulation of the enzyme activity; and (c) mixtures of apoenzyme and active enzyme coexist in the melanosomes.
摘要
  1. 食用蛙的肝脏色素细胞构成了一个具有组织细胞性质的特殊色素细胞系统,并且在黑素小体的蛋白质成分中含有一种类似酪氨酸酶的活性。2. 研究了添加和/或去除铜对该系统多巴氧化酶活性的影响。3. 得出以下结论:(a) 这种酪氨酸酶表现为一种铜酶;(b) 铜可能参与酶活性的调节;(c) 脱辅基酶和活性酶的混合物共存于黑素小体中。

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