Li Jifeng, Zhao Hui, Luo Pengbo, Gu Yudong
Lab of Hand function reconstruction, Huashan Hospital, Fudan University, Shanghai, China.
J Brachial Plex Peripher Nerve Inj. 2010 Dec 7;5:18. doi: 10.1186/1749-7221-5-18.
There is considerable evidence that central nervous system is continuously modulated by activity, behavior and skill acquisition. This study is to examine the reorganization in cortical and subcortical regions in response to brachial plexus avulsion.
Adult C57BL/6 mice were divided into four groups: control, 1, 3 and 6 month of brachial plexus avulsion. IL-1β, IL-6 and RGS4 expression in cortex, brainstem and spinal cord were detected by BiostarM-140 s microarray and real-time PCR. RGS4 subcellular distribution and modulation were further analyzed by primary neuron culture and Western Blot.
After 1, 3 and 6 months of brachial plexus avulsion, 49 (0 up, 49 down), 29 (17 up, 12 down), 13 (9 up, 4 down) genes in cerebral cortex, 40 (8 up, 32 down), 11 (7 up, 4 down), 137 (63 up, 74 down) in brainstem, 27 (14 up, 13 down), 33 (18 up, 15 down), 60 (29 up, 31 down) in spinal cord were identified. Among the regulated gene, IL-1β and IL-6 were sustainable enhanced in brain stem, while PKACβ and RGS4 were up-regulated throughout cerebral cortex, brainstem and spinal cord in 3 and 6 month of nerve injury. Intriguingly, subcellular distribution of RGS4 in above three regions was dependent on the functional correlation of PKA and IL-1β.
Data herein indicated that brachial plexus avulsion could efficiently initiate and perpetuate the brain reorganization. Network involved IL-1β and RGS4 signaling might implicate in the re-establish and strengthening of the local circuits at the cortical and subcortical levels.
有大量证据表明，中枢神经系统会受到活动、行为和技能习得的持续调节。本研究旨在探究臂丛神经撕脱后皮质和皮质下区域的重组情况。
将成年C57BL/6小鼠分为四组：对照组、臂丛神经撕脱1个月、3个月和6个月组。通过BiostarM - 140 s基因芯片和实时PCR检测皮质、脑干和脊髓中白细胞介素 - 1β（IL - 1β）、白细胞介素 - 6（IL - 6）和RGS4的表达。通过原代神经元培养和蛋白质免疫印迹进一步分析RGS4的亚细胞分布及调节情况。
臂丛神经撕脱1个月、3个月和6个月后，大脑皮质中分别有49个（0个上调，49个下调）、29个（17个上调，12个下调）、13个（9个上调，4个下调）基因被鉴定；脑干中分别有40个（8个上调，32个下调）、11个（7个上调，4个下调）、137个（63个上调，74个下调）基因被鉴定；脊髓中分别有27个（14个上调，13个下调）、33个（18个上调，15个下调）、60个（29个上调，31个下调）基因被鉴定。在这些被调节的基因中，IL - 1β和IL - 6在脑干中持续增强，而蛋白激酶A催化亚基β（PKACβ）和RGS4在神经损伤3个月和6个月时在整个大脑皮质、脑干和脊髓中均上调。有趣的是，RGS4在上述三个区域的亚细胞分布取决于蛋白激酶A（PKA）和IL - 1β的功能相关性。
本文数据表明臂丛神经撕脱可有效启动并维持大脑重组。涉及IL - 1β和RGS4信号的网络可能参与了皮质和皮质下水平局部回路的重新建立和强化。
