[超抗原SEA对K562细胞刺激的脐血单个核细胞CD3ε链表达的影响]

[Effect of superantigen SEA on the CD3epsilon chain expression on cord blood mononuclear cells stimulated by K562 cells].

作者信息

Tian Hong-Xia, Gao Yong-Peng, Lin Chen, Chen Shao-Hua, Yang Li-Jian, Li Yang-Qiu

机构信息

Department of Microbiology and Immunology, Medical College, Jinan University, Guangzhou 510632, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Dec;26(12):1175-7, 1181.

PMID:21138676

Abstract

AIM

To explore the effect of staphylococcal enterotoxin A(SEA) on CD3(epsilon) chain expression on mononuclear cells in cord blood, which were stimulated by K562 cells.

METHODS

The anti-CD3 antibodies(mAb), K562 cells, SEA or both SEA and K562 cells were cocultured with mononuclear cells (MNCs) from four normal human cord blood for 48 hours respectively, Real-time PCR with SYBR Green I technique was used to detect expressed level of CD(epsilon) on MNCs in cord blood and set up a blank control group. Relative changes in expression level of CD3(epsilon) chain were indicated by the 2(-deltadeltaCt); method between each group and the control group. beta2-microglobulin gene(beta2M) was used as an endogenous reference.

RESULTS

The expressed level of CD3(epsilon) chain on MNCs was slightly decreased in K562 cell group and were increased in the anti-CD3 antibodies mAb group, SEA group, both of SEA and K562 cell group respectively. The expressed level of CD3(epsilon) chain in both SEA and K562 group was significantly higher than that in the SEA group(P<0.01).

CONCLUSION

Superantigen SEA can enhance the expressed level of CD3(epsilon) chain on MNCs stimulated by K562 cells.

摘要

目的

探讨葡萄球菌肠毒素A（SEA）对K562细胞刺激的脐血单个核细胞CD3（ε）链表达的影响。

方法

将抗CD3抗体（单克隆抗体）、K562细胞、SEA或SEA与K562细胞分别与4份正常人脐血的单个核细胞（MNCs）共培养48小时，采用SYBR Green I技术的实时荧光定量PCR检测脐血MNCs上CD（ε）的表达水平，并设空白对照组。用2^（-ΔΔCt）法表示每组与对照组之间CD3（ε）链表达水平的相对变化。β2-微球蛋白基因（β2M）作为内参。