Lu Xiaoye, Chen Shaohua, Yang Lijian, Zha Xianfeng, Yang Xuesong, Li Yangqiu
Department of Histology & Embryology, Medical College, Jinan University, Guangzhou, China.
Hematology. 2011 May;16(3):185-9. doi: 10.1179/102453311X12953015767419.
In order to investigate the features of T-cell immune status in human placenta, the expression levels of CD3-gamma, -delta, -epsilon and -zeta chain genes in placenta were analyzed by real-time PCR. Umbilical cord blood obtained at delivery from the full-term healthy babies was used as a control. The beta2-microglobulin gene was employed as an endogenous reference, and the evaluations of mRNA expression level of each CD3 gene were used by the 2(-ΔC(t))×100% method. The expression level of CD3-gamma, -epsilon and -zeta genes (mean rank is 0.13, 0.34, and 0.49 respectively) from placenta were significantly lower than those from CB (P<0.0001). CD3-delta genes (mean rank is 5.71) expressed stronger from placenta than from CB (P = 0.0.895). Thus, the expression pattern of the four CD3 genes was presented as delta>zeta>epsilon> gamma from placenta and epsilon>gamma>zeta>delta from CB on the contrary. In conclusion, the present study characterized the expression pattern of CD3-gamma, -delta, -epsilon and -zeta chain genes from placenta, which contributes to further understanding of the features of T-cell immune status in placenta.
为了研究人胎盘组织中T细胞免疫状态的特征，采用实时荧光定量PCR技术分析胎盘组织中CD3-γ、-δ、-ε和-ζ链基因的表达水平。选取足月健康婴儿分娩时的脐带血作为对照。以β2-微球蛋白基因作为内参基因，采用2(-ΔC(t))×100%法对各CD3基因的mRNA表达水平进行评估。胎盘组织中CD3-γ、-ε和-ζ基因的表达水平（平均秩次分别为0.13、0.34和0.49）显著低于脐带血（P<0.0001）。胎盘组织中CD3-δ基因的表达（平均秩次为5.71）强于脐带血（P = 0.0895）。因此，胎盘组织中4种CD3基因的表达模式为δ>ζ>ε>γ，而脐带血中则相反，为ε>γ>ζ>δ。综上所述，本研究明确了胎盘组织中CD3-γ、-δ、-ε和-ζ链基因的表达模式，有助于进一步了解胎盘组织中T细胞免疫状态的特征。
