胆固醇酯在高密度脂蛋白和富含三酰甘油的颗粒之间的可逆流动受三酰甘油的脂肪酸组成和浓度的调节。

Reversible flow of cholesteryl ester between high-density lipoproteins and triacylglycerol-rich particles is modulated by the fatty acid composition and concentration of triacylglycerols.

机构信息

Universidade de São Paulo, SP, Brasil.

出版信息

Braz J Med Biol Res. 2010 Dec;43(12):1135-42. doi: 10.1590/s0100-879x2010007500136. Epub 2010 Dec 3.

DOI:10.1590/s0100-879x2010007500136

Abstract

We determined the influence of fasting (FAST) and feeding (FED) on cholesteryl ester (CE) flow between high-density lipoproteins (HDL) and plasma apoB-lipoprotein and triacylglycerol (TG)-rich emulsions (EM) prepared with TG-fatty acids (FAs). TG-FAs of varying chain lengths and degrees of unsaturation were tested in the presence of a plasma fraction at d > 1.21 g/mL as the source of CE transfer protein. The transfer of CE from HDL to FED was greater than to FAST TG-rich acceptor lipoproteins, 18% and 14%, respectively. However, percent CE transfer from HDL to apoB-containing lipoproteins was similar for FED and FAST HDL. The CE transfer from HDL to EM depended on the EM TG-FA chain length. Furthermore, the chain length of the monounsaturated TG-containing EM showed a significant positive correlation of the CE transfer from HDL to EM (r = 0.81, P < 0.0001) and a negative correlation from EM to HDL (r = -041, P = 0.0088). Regarding the degree of EM TG-FAs unsaturation, among EMs containing C18, the CE transfer was lower from HDL to C18:2 compared to C18:1 and C18:3, 17.7%, 20.7%, and 20%, respectively. However, the CE transfer from EMs to HDL was higher to C18:2 than to C18:1 and C18:3, 83.7%, 51.2%, and 46.3%, respectively. Thus, the EM FA composition was found to be the rate-limiting factor regulating the transfer of CE from HDL. Consequently, the net transfer of CE between HDL and TG-rich particles depends on the specific arrangement of the TG acyl chains in the lipoprotein particle core.

摘要

我们确定了禁食（FAST）和进食（FED）对富含胆固醇酯（CE）的高密度脂蛋白（HDL）与血浆载脂蛋白 B-脂蛋白和富含三酰甘油（TG）的乳剂（EM）之间 CE 流的影响，这些 EM 是用 TG-脂肪酸（FA）制备的。在存在血浆级分（d>1.21 g/mL）作为 CE 转移蛋白来源的情况下，测试了具有不同链长和不饱和度的 TG-FA。从 HDL 到 FED 的 CE 转移大于到 FAST TG 丰富的受体脂蛋白，分别为 18%和 14%。然而，从 HDL 到载脂蛋白 B 含有脂蛋白的 CE 转移百分比在 FED 和 FAST HDL 之间相似。从 HDL 到 EM 的 CE 转移取决于 EM TG-FA 链长。此外，单不饱和 TG 含量的 EM 的链长与从 HDL 到 EM 的 CE 转移呈显著正相关（r=0.81，P<0.0001），与从 EM 到 HDL 的转移呈负相关（r=-0.41，P=0.0088）。关于 EM TG-FA 的不饱和度程度，在含有 C18 的 EM 中，CE 从 HDL 到 C18:2 的转移低于 C18:1 和 C18:3，分别为 17.7%、20.7%和 20%。然而，从 EM 到 HDL 的 CE 转移对 C18:2 高于 C18:1 和 C18:3，分别为 83.7%、51.2%和 46.3%。因此，发现 EM FA 组成是调节 CE 从 HDL 转移的限速因素。因此，HDL 和富含 TG 颗粒之间的 CE 净转移取决于脂蛋白颗粒核心中 TG 酰基链的特定排列。