验证的高效薄层色谱法用于定量测定杨梅科毛杨梅布赫-汉姆（Buch.-Ham.） ex D.唐（D. Don）茎皮中的没食子酸。

Validated HPTLC method for quantitative determination of gallic acid in stem bark of Myrica esculenta Buch.-Ham. ex D. Don, myricaceae.

作者信息

Patel Kalpana G, Patel Vandana G, Patel Kirti V, Gandhi Tejal R

机构信息

Anand Pharmacy College, Shri Ram Krishna Seva Mandal Campus, Near Town Hall, Anand, Gujarat, India.

出版信息

J AOAC Int. 2010 Sep-Oct;93(5):1422-7.

PMID:21140652

Abstract

A simple, rapid, and precise HPTLC method was developed for quantitative estimation of gallic acid in stem bark of Myrica esculenta, family Myricaceae. Separation was performed on silica gel 60F254 HPTLC plates using toluene-ethyl acetate-formic acid-methanol (3 + 3 + 0.6 + 0.4, v/v/v/v) mobile phase for separation of the extracted components. The determination was carried out in the UV densitometric absorbance-reflection mode at 280 nm. The amount of gallic acid in free and combined form in the stem bark powder was found to be 0.276 and 0.541%, respectively, on a dry weight basis. The method was validated in terms of linearity, accuracy, precision, and specificity according to International Conference on Harmonization guidelines. Gallic acid response was found to be linear over a broad concentration range of 0.4-2.0 microg/band. LOD and LOQ were 0.103 and 0.312 microg/spot, respectively. The developed method is capable of quantifying amounts of gallic acid in stem bark powder of M. esculenta.

摘要

开发了一种简单、快速且精确的高效薄层色谱（HPTLC）方法，用于定量测定杨梅科植物毛杨梅茎皮中的没食子酸。采用硅胶60F254 HPTLC板进行分离，以甲苯 - 乙酸乙酯 - 甲酸 - 甲醇（3 + 3 + 0.6 + 0.4，v/v/v/v）为流动相分离提取物中的成分。在280 nm波长下采用紫外光密度计吸光度 - 反射模式进行测定。以干重计，茎皮粉末中游离态和结合态没食子酸的含量分别为0.276%和0.541%。根据国际协调会议指南，该方法在线性、准确度、精密度和特异性方面进行了验证。没食子酸在0.4 - 2.0 μg/条带的宽浓度范围内响应呈线性。检测限（LOD）和定量限（LOQ）分别为0.103和0.312 μg/斑点。所开发的方法能够定量测定毛杨梅茎皮粉末中没食子酸的含量。