[孕酮对新生大鼠缺氧缺血后大脑中葡萄糖转运蛋白（GLUT）表达的影响]
[Effect of progesterone on the expression of GLUT in the brain following hypoxic-ischemia in newborn rats].
作者信息
Li Dong-Liang, Han Hua
机构信息
Department of Physiology and Neurobiology, Xinxiang Medical College, Xinxiang 453003, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2008 Aug;24(3):353-5.
AIM
To investigate the expression of GLUT1 and GLUT3 in the hippocampus after cerebral hypoxic-ischemia (HI) in newborn rats and the effect of progesterone (PROG) on them.
METHODS
Forty newborn SD rats were randomly divided into four groups: normal group, sham-operated group, hypoxic-ischemic group and progesterone group. Model of hypoxic-ischemia encephalopathy (HIE) was established in the 7-day-old newborn SD rats. Immunohistochemical method was applied to detect the expression of GLUT1 and GLUT3 in hippocampus.
RESULTS
GLUT1 and GLUT3 were slightly seen in normal and sham operation group, there was no obviously difference between the two groups (P > 0.05). The expression of GLUT1 and GLUT3 in hypoxic-ischemia group were all higher than that in sham operated group (P < 0.05). Not only the expression of GLUT in progesterone group were significantly higher than that in sham operated group (P < 0.01), but also than that in hypoxic-ischemia group (P < 0.05).
CONCLUSION
PROG could increase the tolerance of neuron to hypoxic-ischemia with maintaining the energy supply in the brain by up-regulating GLUT expression.
目的
探讨新生大鼠脑缺氧缺血（HI）后海马中葡萄糖转运蛋白1（GLUT1）和葡萄糖转运蛋白3（GLUT3）的表达以及孕酮（PROG）对其的影响。
方法
将40只新生SD大鼠随机分为四组：正常组、假手术组、缺氧缺血组和孕酮组。在7日龄新生SD大鼠中建立缺氧缺血性脑病（HIE）模型。采用免疫组织化学方法检测海马中GLUT1和GLUT3的表达。
结果
正常组和假手术组中GLUT1和GLUT3表达较少，两组间无明显差异（P>0.05）。缺氧缺血组中GLUT1和GLUT3的表达均高于假手术组（P<0.05）。孕酮组中GLUT的表达不仅显著高于假手术组（P<0.01），也高于缺氧缺血组（P<0.05）。
结论
PROG可通过上调GLUT表达维持脑内能量供应，从而提高神经元对缺氧缺血的耐受性。
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