Integrated Systems Toxicology Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, North Carolina 27711, USA.
Nanotoxicology. 2011 Dec;5(4):546-56. doi: 10.3109/17435390.2010.539711. Epub 2010 Dec 13.
Six TiO₂ and two CeO₂ nanomaterials with dry sizes ranging from 6-410 nm were tested for their ability to cause DNA centered free radicals in vitro in the concentration range of 10-3,000 ug/ml. All eight of the nanomaterials significantly increased the adduction of the spin trap agent 5,5-dimethyl-1-pyroline N-oxide (DMPO) to DNA as measured by the experimental technique of immuno-spin trapping. The eight nanomaterials differed considerably in their potency, slope, and active concentration. The largest increase in DNA nitrone adducts was caused by a TiO₂ nanomaterial (25 nm, anatase) from Alfa Aesar. Some nanomaterials that increased the amount of DNA nitrone adducts at the lowest exposure concentrations (100 ug/ml) were Degussa TiO₂ (31 nm), Alfa Aesar TiO₂ (25 nm, anatase) and Nanoamor CeO₂ (8 nm, cerianite). At exposure concentrations of 10 or 30 ug/ml, no nanomaterials showed significant in vitro formation of DNA nitrone adducts.
六种 TiO₂ 和两种 CeO₂ 纳米材料，其干粒径范围为 6-410nm，在 10-3000ug/ml 的浓度范围内，测试其在体外引发以 DNA 为中心的自由基的能力。所有这 8 种纳米材料都显著增加了自旋捕获剂 5,5-二甲基-1-吡咯啉 N-氧化物（DMPO）与 DNA 的加合物，这是通过免疫自旋捕获实验技术测量的。这 8 种纳米材料的效力、斜率和有效浓度差异很大。最大的 DNA 硝酮加合物增加是由 Alfa Aesar 的一种 TiO₂ 纳米材料（25nm，锐钛矿）引起的。一些在最低暴露浓度（100ug/ml）下增加 DNA 硝酮加合物数量的纳米材料是 Degussa TiO₂（31nm）、Alfa Aesar TiO₂（25nm，锐钛矿）和 Nanoamor CeO₂（8nm，富铈）。在暴露浓度为 10 或 30ug/ml 时，没有纳米材料显示出体外 DNA 硝酮加合物的显著形成。
