College of Life Sciences and Biotechnology, Korea University, 1, 5-ka, Anam-dong, Sungbuk-ku, Seoul, Korea.
Expert Rev Proteomics. 2010 Dec;7(6):849-66. doi: 10.1586/epr.10.87.
Although accumulating proteomic analyses have supported the fact that mitochondrial oxidative phosphorylation (OXPHOS) complexes are localized in lipid rafts, which mediate cell signaling, immune response and host-pathogen interactions, there has been no in-depth study of the physiological functions of lipid-raft OXPHOS complexes. Here, we show that many subunits of OXPHOS complexes were identified from the lipid rafts of human adipocytes, C2C12 myotubes, Jurkat cells and surface biotin-labeled Jurkat cells via shotgun proteomic analysis. We discuss the findings of OXPHOS complexes in lipid rafts, the role of the surface ATP synthase complex as a receptor for various ligands and extracellular superoxide generation by plasma membrane oxidative phosphorylation complexes.
尽管越来越多的蛋白质组学分析支持线粒体氧化磷酸化（OXPHOS）复合物定位于质膜筏，介导细胞信号转导、免疫反应和宿主-病原体相互作用的事实，但对质膜筏 OXPHOS 复合物的生理功能还没有深入研究。在这里，我们通过鸟枪法蛋白质组学分析显示，人脂肪细胞、C2C12 肌管、Jurkat 细胞和表面生物素标记的 Jurkat 细胞的质膜筏中鉴定出许多 OXPHOS 复合物的亚基。我们讨论了 OXPHOS 复合物在质膜筏中的发现、表面 ATP 合酶复合物作为各种配体的受体以及质膜氧化磷酸化复合物产生细胞外超氧化物的作用。
