Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, OH, USA.
Equine Vet J. 2011 Jan;43(1):54-61. doi: 10.1111/j.2042-3306.2010.00122.x.
There is a need to assess the laminar inflammatory response in a laminitis model that more closely resembles clinical cases of sepsis-related laminitis than the black walnut extract (BWE) model.
To determine if a similar pattern of laminar inflammation, characterised by proinflammatory cytokine expression, occurs in the CHO model of laminitis as has been previously reported for the BWE model.
Sixteen horses administered 17.6 g of starch (85% corn starch/15% wood flour)/kg bwt via nasogastric (NG) tube were anaesthetised either after developing a temperature>38.9°C (DEV group, n=8) or at onset of Obel grade 1 lameness (OG1 group, n=8). Control horses (CON group, n=8) were anaesthetised 24 h after NG administration of 6 l of deionised water. Laminar tissue was collected from horses while under anaesthesia, followed by humane euthanasia. Real time-quantitative PCR was used to assess laminar mRNA concentrations of genes involved in inflammatory signalling.
Increased mRNA concentrations (P<0.05) for IL-1β, IL-6, IL-12p35, COX-2, E-selectin and ICAM-1 were present in laminae from horses with OG1 lameness but not at the DEV time, when compared to the CON horses. No differences between the groups were found for IL-2, IL-4, IL-10, TNF-α, IFN-γ or COX-1 at either the DEV or OG1 time points.
There was a notable difference in the temporal pattern of inflammatory events between the BWE and CHO models, with the majority of laminar inflammatory events appearing to occur at or near the onset of lameness in the CHO model, whereas many of these events peak earlier in the developmental stages in the BWE model. This suggests that, in addition to circulating inflammatory molecules, there may be a local phenomenon in the CHO model resulting in the simultaneous onset of multiple laminar events including endothelial activation, leucocyte emigration and proinflammatory cytokine expression.
The similar (although somewhat delayed) inflammatory response in the CHO model of laminitis indicates that inflammatory signalling is a consistent entity in the pathophysiology of laminitis.
需要评估一种更接近与脓毒症相关的蹄叶炎临床病例的蹄叶炎模型中的层状炎症反应，而不是黑胡桃提取物 (BWE) 模型。
确定在 CHO 蹄叶炎模型中是否会出现类似的层状炎症模式，其特征是促炎细胞因子表达，如以前在 BWE 模型中报道的那样。
通过鼻胃管向 16 匹马施用 17.6 g/kg bwt 的淀粉（85%玉米淀粉/15%木粉），然后对出现体温>38.9°C 的马（DEV 组，n=8）或在 Obel 1 级跛行开始时进行麻醉（OG1 组，n=8）。对照组（CON 组，n=8）在通过鼻胃管给予 6 升去离子水后 24 小时进行麻醉。在麻醉下从马身上采集层状组织，然后进行人道安乐死。实时定量 PCR 用于评估参与炎症信号的基因的层状 mRNA 浓度。
与 CON 马相比，在 OG1 跛行的马的层状中，IL-1β、IL-6、IL-12p35、COX-2、E-选择素和 ICAM-1 的 mRNA 浓度升高（P<0.05），但在 DEV 时间时则没有。在 DEV 或 OG1 时间点，各组之间的 IL-2、IL-4、IL-10、TNF-α、IFN-γ或 COX-1 均无差异。
BWE 和 CHO 模型之间的炎症事件的时间模式有明显差异，CHO 模型中的大多数层状炎症事件似乎发生在跛行开始时或附近，而在 BWE 模型中，许多炎症事件在发育阶段更早达到峰值。这表明，除了循环炎症分子外，CHO 模型中可能存在局部现象，导致多个层状事件同时发生，包括内皮激活、白细胞迁移和促炎细胞因子表达。
CHO 蹄叶炎模型中相似（尽管有些延迟）的炎症反应表明，炎症信号是蹄叶炎病理生理学中的一个一致实体。
