Li L-Y, Li E-M, Wu Z-Y, Huang X, Shen J-H, Xu X-E, Wu J-Y, Huang Q, Xu L-Y
Institute of Oncologic PathologyDepartment of Biochemistry and Molecular Biology, Medical College of Shantou University; and Departments ofOncology SurgeryPathology, Shantou Central Hospital, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou, Guangdong, China.
Dis Esophagus. 2011 Jul;24(5):337-45. doi: 10.1111/j.1442-2050.2010.01147.x. Epub 2010 Dec 10.
Connective tissue growth factor (CTGF, CCN2), a secreted protein, is involved in the development and progression of esophageal squamous cell carcinoma (ESCC). However, it remains unclear how CTGF expression affects the progression of ESCC. Our study implicated differences of CTGF protein status in precancerous lesions, and retrospectively examined the associations of CTGF mRNA and protein levels with clinical prognosis in ESCC patients. Here immunohistochemistry and the quantitative real-time real-time reverse transcription polymerase were performed for predicting the CTGF protein status and mRNA levels in ESCC patients, respectively. Different degrees of CTGF protein status presented in normal human esophageal epithelium and precancerous lesions, and CTGF protein was highly expressed in ESCCs. Survival analysis showed that CTGF protein status was significantly related to poor survival of ESCC patients (P= 0.024), while no significant difference was observed between CTGF mRNA levels and the survival of ESCC patients (P= 0.196). Multivariate Cox analysis demonstrated that CTGF protein status was the independent factor in prognosis of ESCC patients. In that way, CTGF protein status might elevate the progression of ESCC, and would be significant for the diagnosis of precancerous lesions or early ESCC.
结缔组织生长因子（CTGF，CCN2）是一种分泌蛋白，参与食管鳞状细胞癌（ESCC）的发生和发展。然而，CTGF表达如何影响ESCC的进展仍不清楚。我们的研究揭示了癌前病变中CTGF蛋白状态的差异，并回顾性研究了ESCC患者中CTGF mRNA和蛋白水平与临床预后的相关性。在此，分别采用免疫组织化学和定量实时逆转录聚合酶链反应来预测ESCC患者的CTGF蛋白状态和mRNA水平。在正常人食管上皮和癌前病变中呈现出不同程度的CTGF蛋白状态，且CTGF蛋白在ESCC中高表达。生存分析表明，CTGF蛋白状态与ESCC患者的不良生存显著相关（P = 0.024），而CTGF mRNA水平与ESCC患者的生存之间未观察到显著差异（P = 0.196）。多变量Cox分析表明，CTGF蛋白状态是ESCC患者预后的独立因素。因此，CTGF蛋白状态可能促进ESCC的进展，对癌前病变或早期ESCC的诊断具有重要意义。
