Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, Kolkata, India.
Plant Biol (Stuttg). 2011 Jan;13(1):28-35. doi: 10.1111/j.1438-8677.2010.00340.x.
Transcription regulation by the phytohormone abscisic acid (ABA) plays a key role in responses to various stresses and has been extensively studied in Arabidopsis thaliana using different expression analysis techniques. Previous studies on comparative analysis of expression regulation by ABA and ABA-related stresses demonstrated marked differences in the targets of ABA. However, as few expression experiments were available during these studies, they encompass only a subset of ABA-regulated genes. The aim of the present work was to compare gene expression regulated by ABA administration in a wider range of expression experiments. Despite diversity in the actual set of genes regulated by ABA, all ABA-regulated transcriptomes shared some basic features, such as a common induced gene set, common functional targets and involvement of common regulatory mechanisms. Moreover, the cis-regulatory architecture of genes belonging to this set is expected to be of interest because of their sensitivity to ABA and apparent independence from other experimental variables. Comparative analysis of cis-regulatory element (CRE) enrichment also identified a novel CRE that is strongly associated with ABA signalling-mediated transcription repression.
植物激素脱落酸 (ABA) 的转录调控在应对各种胁迫中起着关键作用，并且已经在拟南芥中使用不同的表达分析技术进行了广泛研究。先前关于 ABA 和与 ABA 相关胁迫的表达调控的比较分析表明，ABA 的靶标存在显著差异。然而，由于在这些研究中可用的表达实验较少，它们仅涵盖了 ABA 调节基因的一部分。本工作的目的是比较 ABA 给药在更广泛的表达实验中调节的基因表达。尽管 ABA 调节的转录组在实际受调控的基因集合上存在多样性，但所有 ABA 调节的转录组都具有一些共同的基本特征，例如共同的诱导基因集、共同的功能靶标和共同的调控机制的参与。此外，由于它们对 ABA 的敏感性和明显独立于其他实验变量，属于该集合的基因的顺式调控结构预计将是有趣的。顺式调控元件 (CRE) 富集的比较分析还鉴定了一个与 ABA 信号转导介导的转录抑制强烈相关的新 CRE。
