Department of Orthopedic Surgery, Orthopedics Oncology Institute of Chinese PLA, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, 710038, P.R. China.
J Exp Clin Cancer Res. 2010 Dec 8;29(1):158. doi: 10.1186/1756-9966-29-158.
Chordoma was a typically slow-growing tumor. The therapeutic approach to chordoma had traditionally relied mainly on surgical therapy. And the main reason for therapeutic failure was resistance to chemotherapy and radiotherapy. However the refractory mechanism was not clear. The aim of this study was to investigate the expression of three genes (MDR1, HIF-1α and MRP1) associated with resistance to chemotherapy and radiotherapy in chordoma and chordoma cell line CM-319.
Using immunohistochemical techniques, the expression of MDR1, HIF-1α and MRP1 was investigated in 50 chordoma specimen. Using RT-PCR and Western blot, the expression of MDR1, HIF-1α and MRP1 was investigated in chordoma and chordoma cell line CM-319.
Expression of MDR1, HIF-1α and MRP1 was observed in 10%, 80% and 74% of all cases, respectively. Expression of MRP1 was correlated with HIF-1α. On the other hand, expression of MDR1 was not correlated with the expression of HIF-1α or MRP1. The expression of HIF-1α and MRP1 was observed, but MDR1 was not observed in chordoma and CM-319.
Expression of HIF-1α and MRP1 was observed in most chordoma specimen and CM-319 cell line; expression of HIF-1α correlated with MRP1. HIF-1α and MRP1 may play a role in the multidrug resistance of chordoma to chemotherapy.
软骨肉瘤是一种典型的生长缓慢的肿瘤。软骨肉瘤的治疗方法传统上主要依赖于手术治疗。而治疗失败的主要原因是对化疗和放疗的耐药性。然而，耐药机制尚不清楚。本研究旨在探讨与化疗和放疗耐药相关的三个基因（MDR1、HIF-1α 和 MRP1）在软骨肉瘤和软骨肉瘤细胞系 CM-319 中的表达。
采用免疫组织化学技术，检测 50 例软骨肉瘤标本中 MDR1、HIF-1α 和 MRP1 的表达。采用 RT-PCR 和 Western blot 检测软骨肉瘤和软骨肉瘤细胞系 CM-319 中 MDR1、HIF-1α 和 MRP1 的表达。
MDR1、HIF-1α 和 MRP1 的表达分别在所有病例的 10%、80%和 74%中观察到。MRP1 的表达与 HIF-1α 相关。另一方面，MDR1 的表达与 HIF-1α 或 MRP1 的表达无关。在软骨肉瘤和 CM-319 中观察到 HIF-1α 和 MRP1 的表达，但未观察到 MDR1 的表达。
大多数软骨肉瘤标本和 CM-319 细胞系中观察到 HIF-1α 和 MRP1 的表达；HIF-1α 的表达与 MRP1 相关。HIF-1α 和 MRP1 可能在软骨肉瘤对化疗的多药耐药中发挥作用。
