Zhang Xiuwei, Qi Fengjie, Dai Shundong, Wang Enhua
Department of Pathology, College of Basic Medical Science, China Medical University, Shenyang, Liaoning 110001, P.R.China.
Zhongguo Fei Ai Za Zhi. 2006 Feb 20;9(1):45-9. doi: 10.3779/j.issn.1009-3419.2006.01.12.
So-called pulmonary sclerosing hemangioma (PSH) is a rare kind of pulmonary tumor. Its histological origin and nature, which have become research hot spots for many years, are still uncertain. The aim of this study is to investigate the immunophenotype of cuboidal cells and polygonal cells through observing the expression of E-cadherin, β-catenin and p120ctn in cuboidal cells and polygonal cells of PSH.
Expression of E-cadherin, β-catenin and p120 ctn was detected in 25 cases of PSH samples and 8 cases of pulmonary inflammatory pseudotumor samples by immunohistochemistry.
Immunohistochemistry results showed that the surface cuboidal cells of PSH were strongly positive on membrane for E-cadherin, β-catenin and p120 ctn , with cytoplasmic positive expression of β-catenin. However,the polygonal cells were negative for E-cadherin, cytoplasmic positive for β-catenin and predominantly cytoplasmic positive and weakly membranous positive for p120ctn . In polygonal cells, all the three adhesion molecules showed heterogenicity staining. The cytoplasm and membrane of inner covering cells in the hemorrhagic pattern were positively stained for E-cadherin, β-catenin and p120ctn . The expression of the three adhesion molecules in hyperplastic type II alveolar cells of pulmonary inflammatory pseudotumor was similar to cuboidal cells of PSH.
It is concluded that cuboidal cells of PSH may be the hyperplastic type II alveolar cells, whereas polygonal cells are true tumor cells lacking the E-cadherin/catenin complex which is expressed in well differentiated epithelial cells. The inner covering cells in the hemorrhagic pattern of PSH are perhaps epithelial cells as cuboidal cells rather than vascular endothelial cells.
所谓的肺硬化性血管瘤（PSH）是一种罕见的肺肿瘤。其组织学起源和性质多年来一直是研究热点，至今仍不明确。本研究旨在通过观察E-钙黏蛋白、β-连环蛋白和p120连环蛋白在PSH的立方细胞和多边形细胞中的表达，探讨其免疫表型。
采用免疫组织化学法检测25例PSH标本和8例肺炎性假瘤标本中E-钙黏蛋白、β-连环蛋白和p120连环蛋白的表达。
免疫组织化学结果显示，PSH的表面立方细胞E-钙黏蛋白、β-连环蛋白和p120连环蛋白膜强阳性，β-连环蛋白有胞质阳性表达。然而，多边形细胞E-钙黏蛋白阴性，β-连环蛋白胞质阳性，p120连环蛋白主要为胞质阳性且膜弱阳性。在多边形细胞中，所有三种黏附分子均呈异质性染色。出血型内被覆细胞的胞质和膜E-钙黏蛋白、β-连环蛋白和p120连环蛋白呈阳性染色。肺炎性假瘤增生型II型肺泡细胞中三种黏附分子的表达与PSH的立方细胞相似。
得出结论，PSH的立方细胞可能是增生型II型肺泡细胞，而多边形细胞是真正的肿瘤细胞，缺乏在高分化上皮细胞中表达的E-钙黏蛋白/连环蛋白复合体。PSH出血型的内被覆细胞可能是立方细胞样的上皮细胞，而非血管内皮细胞。
