[bcl-2、bax、P53在肺神经内分泌癌中的表达及意义]

[Expression and significance of bcl-2, bax, P53 in neuroendocrine carcinomas of the lung].

作者信息

Shao Yun, Zhao Po, Li Xianghong

机构信息

Department of Pathology, Chinese PLA General Hospital, Beijing 100039, P.R.China.

出版信息

Zhongguo Fei Ai Za Zhi. 2006 Feb 20;9(1):55-9. doi: 10.3779/j.issn.1009-3419.2006.01.14.

DOI:10.3779/j.issn.1009-3419.2006.01.14

PMID:21144283

Abstract

BACKGROUND

bcl-2 and bax are main regulators of cell apoptosis, both are downstream genes of wild-type p53 gene. This study is to investigate the expression of bcl-2, bax, P53, C-erbB-2 and nm23-H1 in neuroendocrine carcinomas of the lung (NEC) and their relationship to tumor biological characteristics and survival time of patients.

METHODS

Hematoxylin-eosin staining and SP immunohistochemical staining were performed on paraffin embedded tissues of 59 patients with NEC. The relationship between the expression of p53, bcl-2, bax and the clinicopathological factors was analyzed by Chi-square test, multivariate analysis and Kaplan-Meier method.

RESULTS

The following positive expression rates were observed in cancer tissue: bax 56%, bcl-2 46%, P53 27%, C-erbB-2 14%, nm23-H1 90%. Of them bcl-2 related to pathological type (P=0.037), TNM stage (P=0.011), and lymph node metastasis (P=0.020), bax didn't relate to these biological characteristics. Both bcl-2 and P53 showed negative correlation to prognosis in Kaplan-Meier survival analysis (P=0.0338 and 0.0375 respectively), the expression of which linked to poor prognosis and short survival time, while nm23-H1 showed positive correlation to prognosis (P=0.0021), but Cox survival analysis demonstrated that only bcl-2 was associated with survival time of patients (P=0.011).

CONCLUSIONS

bcl-2 is an independent prognostic factor in NEC.

摘要

背景

bcl-2和bax是细胞凋亡的主要调节因子，二者均为野生型p53基因的下游基因。本研究旨在探讨bcl-2、bax、P53、C-erbB-2和nm23-H1在肺神经内分泌癌（NEC）中的表达及其与肿瘤生物学特征和患者生存时间的关系。

方法

对59例NEC患者的石蜡包埋组织进行苏木精-伊红染色和SP免疫组织化学染色。采用卡方检验、多因素分析和Kaplan-Meier法分析p53、bcl-2、bax表达与临床病理因素的关系。

结果

癌组织中观察到以下阳性表达率：bax 56%，bcl-2 46%，P53 27%，C-erbB-2 14%，nm23-H1 90%。其中bcl-2与病理类型（P=0.037）、TNM分期（P=0.011）和淋巴结转移（P=0.020）有关，bax与这些生物学特征无关。在Kaplan-Meier生存分析中，bcl-2和P53均与预后呈负相关（分别为P=0.0338和0.0375），其表达与预后不良和生存时间短有关，而nm23-H1与预后呈正相关（P=0.0021），但Cox生存分析表明只有bcl-2与患者生存时间相关（P=0.011）。