Bao Yongxia, Lv Fuzhen, Ma Yingjun
Department of Respiratory Diseases, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R.China.
Zhongguo Fei Ai Za Zhi. 2006 Apr 20;9(2):143-6. doi: 10.3779/j.issn.1009-3419.2006.02.09.
It has been proved that hypoxia is closely related to oncogenesis and development of tumor. The aim of this study is to observe the effect of dexamethasone on expression of hypoxia inducible factor 1α (HIF-1α) and vascular endothelial growth factor (VEGF) in lung tissues of hypoxic mice, and to investigate the relationship between hypoxia and angiogenesis and mechanism of dexamethasone.
The Kunming mice were randomly divided into control group and three experimental groups (3-day hypoxia group, 6-day hypoxia group, and hypoxia+dexamethasone group). HIF-1α and VEGF protein expression was detected in lung tissues of mice by immunohistochemistry.
Expression of HIF-1α and VEGF significantly increased in hypoxia group compared with control group (P < 0.05). Compared with hypoxia group, expression of HIF-1α and VEGF decreased dramatically in hypoxia+dexamethasone group (P < 0.05). A positive correlation was found between the expression of HIF-1α and VEGF (r=0.730, P=0.007).
Hypoxia can increase the expression of VEGF and HIF-1α in lung tissues of mice. Dexamethasone can inhibit the expression of VEGF and HIF-1α of hypoxic mice and it may have anti-angiogenetic effect.
已证实缺氧与肿瘤的发生和发展密切相关。本研究旨在观察地塞米松对缺氧小鼠肺组织中缺氧诱导因子1α（HIF-1α）和血管内皮生长因子（VEGF）表达的影响，并探讨缺氧与血管生成之间的关系以及地塞米松的作用机制。
将昆明小鼠随机分为对照组和三个实验组（3天缺氧组、6天缺氧组和缺氧+地塞米松组）。采用免疫组织化学法检测小鼠肺组织中HIF-1α和VEGF蛋白的表达。
与对照组相比，缺氧组HIF-1α和VEGF的表达显著增加（P<0.05）。与缺氧组相比，缺氧+地塞米松组HIF-1α和VEGF的表达显著降低（P<0.05）。HIF-1α和VEGF的表达之间存在正相关（r=0.730，P=0.007）。
缺氧可增加小鼠肺组织中VEGF和HIF-1α的表达。地塞米松可抑制缺氧小鼠VEGF和HIF-1α的表达，可能具有抗血管生成作用。
