精子质量分析仪（SQA-Vp）在猪精液分析中的应用效果。

Effectiveness of the sperm quality analyzer (SQA-Vp) for porcine semen analysis.

机构信息

Department of Obstetrics, Reproduction and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.

出版信息

Theriogenology. 2011 Mar 15;75(5):972-7. doi: 10.1016/j.theriogenology.2010.10.024. Epub 2010 Dec 7.

DOI:10.1016/j.theriogenology.2010.10.024

PMID:21144570

Abstract

The Sperm Quality Analyzer (SQA-Vp) was evaluated for assessing concentration and motility of porcine semen. Both fresh and diluted semen from 50 different boars from a commercial artificial insemination (AI) centre were investigated. For the fresh ejaculate, the concentration obtained with SQA-Vp was compared with a photometer and a haemocytometer. For the diluted samples, the concentration and motility were compared with computer assisted semen analysis (CASA) and visual sperm analysis. The agreement between methods was studied with Bland-Altman plots and the repeatability with coefficient of variation (CV) as well as Bland-Altman plots. The sperm concentration (x10(6)/ml) obtained with SQA-Vp (379.3 ± 134.9) for fresh ejaculates agreed well with concentration by the photometer (447.2 ± 154.2; difference= -67.9 x 10(6)/ml; difference + 2SD = 55.3 x 10(6)/ml; difference - 2SD = -191.1 x 10(6)/ml) and with the haemocytometer (332.8 ± 141.11; d = 92.8; d + 2SD = 448.6; d - 2SD = -263). For diluted semen, the agreement between the concentration (x10(6)/ml) assessed with SQA-Vp (20.4 ± 4.3) was good with CASA (23.2 ± 5.8; d = -2.8; d + 2SD = 6.2; d - 2 SD = -11.8) but poor with the haemocytometer (18.8 ± 5.0; d = 1.6; d+ 2SD = 12.2; d - 2SD = -9). The % motile spermatozoa assessed by SQA-Vp (65.8 ± 10.0) in diluted semen agreed well with CASA (72.2 ± 13.7; d = -6.4; d+ 2SD = 20; d - 2SD = -32.8) and with visual assessment (64.1 ± 11.6; d = 1.7; d+ 2SD = 30.9; d - 2SD = -27.5). The SQA-Vp showed a good repeatability (CV; repeatability coefficient) for measuring the concentration of both fresh (3.9%; d = 10.7; d + 2SD = 30.9; d - 2SD = -9.5) and diluted semen (2.6%; d = 1.0; d + 2SD = 2.38; d - 2SD = -0.42) and for motility (3.2%; d = 0.9; d + 2SD = 8.5; d - 2SD = -6.7). The mean SQA-Vp values fell between the other methods' results for both fresh and diluted semen. Moreover the repeatability was acceptable. Therefore SQA-Vp can be used as a valid device for sperm quality analysis in pigs.

摘要

精液质量分析仪（SQA-Vp）用于评估猪精液的浓度和活力。从商业人工授精（AI）中心的 50 头不同公猪中采集新鲜和稀释的精液进行研究。对于新鲜精液，SQA-Vp 获得的浓度与光度计和血球计数板进行比较。对于稀释的样本，浓度和活力与计算机辅助精液分析（CASA）和视觉精子分析进行比较。使用 Bland-Altman 图研究方法之间的一致性，并使用变异系数（CV）和 Bland-Altman 图研究重复性。SQA-Vp 获得的精子浓度（x10(6)/ml）（379.3 ± 134.9）与光度计（447.2 ± 154.2）和血球计数板（332.8 ± 141.11）的浓度一致；差异=-67.9 x 10(6)/ml；差异+ 2SD = 55.3 x 10(6)/ml；差异- 2SD = -191.1 x 10(6)/ml）。对于稀释的精液，SQA-Vp（20.4 ± 4.3）评估的浓度与 CASA（23.2 ± 5.8；d = -2.8；d + 2SD = 6.2；d - 2 SD = -11.8）之间的一致性良好，但与血球计数板（18.8 ± 5.0；d = 1.6；d+ 2SD = 12.2；d - 2SD = -9）的一致性较差。SQA-Vp 在稀释精液中评估的活动精子百分比（65.8 ± 10.0）与 CASA（72.2 ± 13.7；d = -6.4；d+ 2SD = 20；d - 2SD = -32.8）和视觉评估（64.1 ± 11.6；d = 1.7；d+ 2SD = 30.9；d - 2SD = -27.5）一致。SQA-Vp 显示出良好的重复性（CV；重复性系数），用于测量新鲜（3.9%；d = 10.7；d + 2SD = 30.9；d - 2SD = -9.5）和稀释（2.6%；d = 1.0；d + 2SD = 2.38；d - 2SD = -0.42）精液的浓度和活力。SQA-Vp 在新鲜和稀释精液中的平均值均介于其他方法的结果之间。此外，重复性可以接受。因此，SQA-Vp 可作为一种有效的猪精液质量分析设备。