Jokinen Päivi, Rusanen Elina M, Kennedy Lorna J, Lohi Hannes
Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Agnes Sjöbergin katu 2, P.O. Box 66, 00014 University of Helsinki, Finland.
Vet Immunol Immunopathol. 2011 Mar 15;140(1-2):37-41. doi: 10.1016/j.vetimm.2010.11.007. Epub 2010 Nov 18.
Canine chronic superficial keratitis (CSK) is an inflammatory ocular disease of an autoimmune origin leading to blindness if untreated. The main symptoms of CSK are progressive, bilateral vascularisation, fibrous tissue formation and pigmentation of the anterior corneal stroma. Although CSK is found in many breeds it is most prevalent in German Shepherd dogs (GSDs). Since Major Histocompatibility Complex (MHC) class II is associated with several autoimmune diseases in dogs we investigated the possible role of DLA-DRB1, -DQA1 and -DQB1 in GSDs affected with CSK. Our study population included 25 healthy controls and 30 CSK dogs. Most of the affected dogs were females suggesting a female predisposition. We identified 11 unevenly distributed haplotypes of which DLA-DRB101501/DQA100601/DQB1*00301 was significantly associated with the CSK dogs (OR=2.67, CI=1.17-6.44, p=0.02). We also found that overall homozygosity of MHC class II increases risk for CSK (OR=4.37, CI=1.27-18.46, p=0.02) and homozygosity of the risk haplotype by over eight-fold (OR=8.5, 95% CI=1.4-224, p=0.017). This study identifies a MHC class II risk haplotype for CSK in GSD and further supports the autoimmune origin of the disease.
犬慢性浅层角膜炎（CSK）是一种自身免疫性起源的炎症性眼病，若不治疗会导致失明。CSK的主要症状是渐进性的双侧血管化、纤维组织形成以及角膜前基质色素沉着。尽管CSK在许多犬种中都有发现，但在德国牧羊犬（GSD）中最为普遍。由于主要组织相容性复合体（MHC）II类与犬的几种自身免疫性疾病有关，我们研究了DLA-DRB1、-DQA1和-DQB1在患有CSK的GSD中的可能作用。我们的研究群体包括25只健康对照犬和30只患有CSK的犬。大多数患病犬为雌性，提示雌性易感性。我们鉴定出11种分布不均的单倍型，其中DLA-DRB101501/DQA100601/DQB1*00301与患有CSK的犬显著相关（OR=2.67，CI=1.17-6.44，p=0.02）。我们还发现，MHC II类的总体纯合性增加了患CSK的风险（OR=4.37，CI=1.27-18.46，p=0.02），风险单倍型的纯合性增加了八倍多（OR=8.5，95%CI=1.4-224，p=0.017）。本研究确定了GSD中CSK的一种MHC II类风险单倍型，并进一步支持了该疾病的自身免疫性起源。
