制作标记区域以对准棒状样品的 TEM 断层倾斜系列。
Fabrication of marker area to align TEM tomographic tilt series of rod-shaped specimens.
机构信息
National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology, Central 5, Higashi 1-1-1, Tsukuba, Ibaraki 305-8565, Japan.
出版信息
Micron. 2011 Apr;42(3):307-10. doi: 10.1016/j.micron.2010.11.004. Epub 2010 Nov 12.
In this study, we present a new method for placing markers for alignment of tomographic tilt-series of rod-shaped specimen before 3D reconstructions. By this method, markers (gold nanoparticles) were placed only on the carbon layer (referred to as the "marker area") deposited for protecting the specimen surface against ion beam irradiation; this placement was achieved by vacuum evaporation of gold with the help of a mask fabricated adjacent to the specimen. Experimental results showed that the use of the proposed method facilitates the identification of the markers in the alignment process, because the image of the marker area consists of simple high-contrast images of the gold nanoparticles on the carbon layer. The performance of the proposed method was successfully verified experimentally by applying it to a high-density Au/SiO₂ nanocomposite material as a test specimen.
在这项研究中，我们提出了一种新的方法，用于在三维重建之前为杆状标本的断层倾斜系列放置标记以进行对准。通过这种方法，仅将标记（金纳米颗粒）放置在沉积用于保护标本表面免受离子束辐照的碳层上（称为“标记区域”）; 这是通过使用与标本相邻制造的掩模辅助的金真空蒸发来实现的。实验结果表明，该方法的使用便于在对准过程中识别标记，因为标记区域的图像由碳层上的金纳米颗粒的简单高对比度图像组成。通过将其应用于高密度 Au/SiO₂纳米复合材料作为测试样品，成功地通过实验验证了该方法的性能。
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