Institute of Biochemistry and Molecular Biology, School of Life Science, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, PR China.
Neurosci Lett. 2011 Feb 4;489(2):131-5. doi: 10.1016/j.neulet.2010.12.002. Epub 2010 Dec 9.
In the present study, we investigated the antidepressive activity of opiorphin with central administration in the forced swim test in mice. Intracerebroventricular (i.c.v.) administration of opiorphin (1-6 μg/mouse) dose-dependently decreased the immobility time, which was reversed by nonselective opioid receptor antagonist naloxone, δ-selective naltrindole and μ-selective β-FNA. The data suggested that central administration of opiorphin produced an antidepressant-like effect by activating both μ and δ opioid receptors indirectly. In order to eliminate the possibility of a false-positive result in the forced swim test, locomotor activity was checked in both non-habituated and habituated mice. Opiorphin had no influence on non-habituated mice, though had weak effect on habituated mice. In addition, mice treated with opiorphin did not display any convulsive behaviors.
在本研究中，我们通过在小鼠强迫游泳试验中中枢给予阿片啡研究其抗抑郁活性。阿片啡（1-6 μg/只小鼠）脑室内给药剂量依赖性地减少不动时间，该作用可被非选择性阿片受体拮抗剂纳洛酮、δ 选择性纳曲吲哚和 μ 选择性 β-FNA 反转。数据表明，阿片啡通过间接激活 μ 和 δ 阿片受体产生抗抑郁样作用。为了排除强迫游泳试验中出现假阳性结果的可能性，在非习惯化和习惯化的小鼠中检查了运动活性。阿片啡对非习惯化的小鼠没有影响，尽管对习惯化的小鼠有微弱的影响。此外，给予阿片啡的小鼠没有出现任何惊厥行为。
