Liudvig M Z, Uspenskiĭ I I, Ivanov A I, Tamarina N A, Korochkin L I
Ontogenez. 1990 Mar-Apr;21(2):133-7.
Tissue localization and synthesis of PEB (the major protein of ejaculatory bulb (EB) in D. melanogaster) were studied. PEB and its minor form hPEB were detected only in EB as shown by immunoblotting technique. Transplantation of genital imaginal disks and the EBs of young males into recipients of strain with different PEB electrophoretic mobility showed that synthesis and accumulation of PEB do not depend on EB interaction with other organs. In genotypic females transformed into males and intersexes by sex-reversing mutations PEB was detected in EB but not in other organs.
研究了PEB(黑腹果蝇射精球(EB)的主要蛋白质)的组织定位和合成。如免疫印迹技术所示,仅在射精球中检测到PEB及其次要形式hPEB。将生殖器成虫盘和年轻雄性的射精球移植到具有不同PEB电泳迁移率的品系受体中,结果表明PEB的合成和积累不依赖于射精球与其他器官的相互作用。在通过性反转突变转化为雄性和雌雄同体的基因型雌性中,在射精球中检测到了PEB,但在其他器官中未检测到。