Department of Oral Biology, School of Dentistry, University of Birmingham, Birmingham, UK.
J Endod. 2011 Jan;37(1):26-30. doi: 10.1016/j.joen.2010.08.042. Epub 2010 Oct 15.
Angiogenesis is key to both the development and regeneration of the dentin-pulp complex.
We hypothesized that proangiogenic signaling molecules sequestered in dentin matrix can be solubilised to induce angiogenic events.
Matrix components were extracted from powdered sound human dentin with EDTA and their dose-dependent (0.0001-5 mg/mL) effects examined in endothelial cells in an in vitro angiogenic tube formation assay, proliferation assay, and transcriptional regulation of the VEGF and VEGF-R2 genes.
Lower concentrations of dentin matrix components were found to show proangiogenic activity, whereas higher concentrations suppressed angiogenic activity.
This study highlights that the release of dentin matrix components after dental injury can contribute to the angiogenic events that support pulp regeneration.
血管生成对于牙本质牙髓复合体的发育和再生都是至关重要的。
我们假设牙本质基质中被隔离的促血管生成信号分子可以被溶解，以诱导血管生成事件。
用 EDTA 从粉末状的健康人牙本质中提取基质成分，并在体外血管生成管形成试验、增殖试验以及 VEGF 和 VEGF-R2 基因的转录调控中，检测其在人内皮细胞中的剂量依赖性（0.0001-5mg/ml）作用。
发现较低浓度的牙本质基质成分具有促血管生成活性，而较高浓度则抑制血管生成活性。
本研究强调了牙损伤后牙本质基质成分的释放可能有助于支持牙髓再生的血管生成事件。
