Division of Pediatric Gastroenterology, Federal University of São Paulo, Escola Paulista de Medicina São Paulo, São Paulo, Brazil.
Division of Pediatric Gastroenterology, Federal University of São Paulo, Escola Paulista de Medicina São Paulo, São Paulo, Brazil.
Nutrition. 2011 Jan;27(1):111-115. doi: 10.1016/j.nut.2010.07.002.
To evaluate the transepithelial transport of sodium, glucose, potassium, and water and the mRNA level of the sodium-glucose cotransporter (SGLT1) and the facilitated sugar transporter (GLUT2) in the small intestine of iron-deficient rats.
After 6 wk of receiving diets with low or normal iron content, rats (Wistar-EPM) were subjected to two experiments: 1) evaluation of the transepithelial transport of sodium, glucose, potassium, and water by an "in vivo" experimental model of intestinal perfusion and 2) determination of relative SGLT1 and GLUT2 mRNA levels in the proximal, intermediate, and distal portions of the small intestine by the northern blotting technique.
Hemoglobin and hepatic iron levels were statistically lower in the anemic rats. The mean transepithelial transports of sodium (-33.0 μEq · min(-1) · cm(-1), glucose (426.0 μM · min(-1) · cm(-1), and water (0.4 μL · min(-1) · cm(-1) in the small intestine of the anemic rats were significantly lower than in the control group (349.1 μEq · min(-1) · cm(-1), 842.6 μM · min(-1) · cm(-1), and 4.3 μl · min(-1) · cm(-1), respectively, P < 0.05). The transepithelial transport of potassium was similar for both groups. The relative SGLT1 mRNA levels of the anemic rats in the intermediate (1.796 ± 0.659 AU) and distal (1.901 ± 0.766 AU) segments were significantly higher than the values for the control rats (intermediate 1.262 ± 0.450 AU, distal 1.244 ± 0.407 AU). No significant difference was observed for the relative SLGT1 mRNA levels in the proximal segment or for the GLUT2 mRNA levels in all segments.
Iron deficiency decreases the absorption of glucose, sodium, and water and increases SGLT1 mRNA in the intermediate and distal segments of the small intestine of rats.
评估铁缺乏大鼠小肠的跨上皮钠离子、葡萄糖、钾和水转运以及钠-葡萄糖协同转运蛋白（SGLT1）和易化性糖转运蛋白（GLUT2）的 mRNA 水平。
接受低铁或正常铁含量饮食 6 周后，大鼠（Wistar-EPM）进行了两项实验：1）通过肠道灌流的“体内”实验模型评估钠离子、葡萄糖、钾和水的跨上皮转运；2）通过 northern 印迹技术测定小肠近端、中段和远端的相对 SGLT1 和 GLUT2 mRNA 水平。
贫血大鼠的血红蛋白和肝铁水平明显降低。贫血大鼠小肠的钠（-33.0 μEq·min-1·cm-1）、葡萄糖（426.0 μM·min-1·cm-1）和水（0.4 μL·min-1·cm-1）的平均跨上皮转运明显低于对照组（349.1 μEq·min-1·cm-1、842.6 μM·min-1·cm-1 和 4.3 μL·min-1·cm-1，P<0.05）。两组的钾跨上皮转运相似。贫血大鼠中段（1.796±0.659 AU）和远端（1.901±0.766 AU）SGLT1 mRNA 相对水平明显高于对照组（中段 1.262±0.450 AU，远端 1.244±0.407 AU）。近端 SGLT1 mRNA 相对水平或各段的 GLUT2 mRNA 水平无显著差异。
铁缺乏降低了大鼠小肠葡萄糖、钠和水的吸收，并增加了中段和远端 SGLT1 mRNA 的表达。
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