Institute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.
Diagn Microbiol Infect Dis. 2011 Jan;69(1):38-44. doi: 10.1016/j.diagmicrobio.2010.09.008.
Acinetobacter baumannii is an emerging nosocomial pathogen that is resistant to many types of antibiotics, and hence, a fast, sensitive, specific, and economical test for its rapid diagnosis is needed. Development of such a test requires a specific antigen, and outer membrane proteins (OMPs) are the prime candidates. The goal of this study was to find a specific OMP of A. baumannii and demonstrate the presence of specific IgM, IgA, and IgG against the candidate protein in human serum. OMPs of A. baumannii ATCC 19606 and 16 other clinical isolates of A. baumannii were extracted from an overnight culture grown at 37 °C. Protein profiles were obtained using sodium dodecyl sulfate polyacrylamide gel electrophoresis, and Western blot analysis was performed to detect the presence of IgM, IgA, and IgG against the OMP in host serum. An antigenic 34.4-kDa OMP was uniquely recognized by IgM, IgA, and IgG from patients with A. baumannii infection, and it did not cross-react with sera from patients with other types of infection. The band was also found in the other 16 A. baumannii isolates. This 34.4-kDa OMP is a prime candidate for development of a diagnostic test for the presence of A. baumannii.
鲍曼不动杆菌是一种新兴的医院获得性病原体，对许多类型的抗生素具有耐药性，因此需要快速、敏感、特异和经济的检测方法来快速诊断。这种检测方法的开发需要特定的抗原，而外膜蛋白（OMPs）是主要的候选物。本研究的目的是寻找鲍曼不动杆菌的特定 OMP，并证明在人血清中存在针对候选蛋白的特异性 IgM、IgA 和 IgG。从在 37°C 下培养过夜的 A. baumannii ATCC 19606 和其他 16 株临床分离株中提取 OMPs。使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳获得蛋白质图谱，并进行 Western blot 分析以检测宿主血清中针对 OMP 的 IgM、IgA 和 IgG 的存在。一种 34.4 kDa 的抗原性 OMP 被感染鲍曼不动杆菌的患者的 IgM、IgA 和 IgG 特异性识别，与来自其他类型感染患者的血清无交叉反应。该条带也存在于其他 16 株鲍曼不动杆菌分离株中。这种 34.4 kDa 的 OMP 是开发用于检测鲍曼不动杆菌存在的诊断试验的主要候选物。
