Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Comp Immunol Microbiol Infect Dis. 2011 May;34(3):227-36. doi: 10.1016/j.cimid.2010.11.006. Epub 2010 Dec 13.
In the present study, we describe the development of a DNA vaccine against chicken anemia virus. The VP1 and VP2 genes of CAV were amplified and cloned into pBudCE4.1 to construct two DNA vaccines, namely, pBudVP1 and pBudVP2-VP1. In vitro and in vivo studies showed that co-expression of VP1 with VP2 are required to induce significant levels of antibody against CAV. Subsequently, the vaccines were tested in 2-week-old SPF chickens. Chickens immunized with the DNA-plasmid pBudVP2-VP1 showed positive neutralizing antibody titer against CAV. Furthermore, VP1-specific proliferation induction of splenocytes and also high serum levels of Th1 cytokines, IL-2 and IFN-γ were detected in the pBudVP2-VP1-vaccinated chickens. These results suggest that the recombinant DNA plasmid co-expressing VP1 and VP2 can be used as a potential DNA vaccine against CAV.
在本研究中，我们描述了一种针对鸡贫血病毒的 DNA 疫苗的开发。CAV 的 VP1 和 VP2 基因被扩增并克隆到 pBudCE4.1 中，构建了两种 DNA 疫苗，即 pBudVP1 和 pBudVP2-VP1。体外和体内研究表明，VP1 与 VP2 的共表达是诱导针对 CAV 的抗体产生的必要条件。随后，在 2 周龄 SPF 鸡中进行了疫苗测试。用 DNA 质粒 pBudVP2-VP1 免疫的鸡表现出针对 CAV 的阳性中和抗体滴度。此外，在 pBudVP2-VP1 免疫的鸡中还检测到了 VP1 特异性脾细胞增殖诱导以及高水平的 Th1 细胞因子 IL-2 和 IFN-γ。这些结果表明，共表达 VP1 和 VP2 的重组 DNA 质粒可用作针对 CAV 的潜在 DNA 疫苗。
