State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, PR China.
Colloids Surf B Biointerfaces. 2011 Apr 1;83(2):220-8. doi: 10.1016/j.colsurfb.2010.11.020. Epub 2010 Nov 18.
Fourier transform surface plasmon resonance (FT-SPR) was utilized to study specific and non-specific interactions between proteins and a biotinylated polymer film by monitoring adsorptions of streptavidin (SAv) and bovine serum albumin (BSA) on the polymer films. The biotinylated polymer, poly(lactide-co-2,2-dihydroxymethyl-propylene carbonate-graft-biotin) [P(LA-co-DHC/biotin)], was prepared by ring-opening copolymerization of lactide and a OH-bearing cyclic carbonate monomer, followed by biotinylation of the OH groups. The copolymer was coated onto the FT-SPR chip and vacuum-dried, hydrated at 70°C, and treated with a blocking agent respectively to achieve different surface status. The FT-SPR results showed that the vacuum-dried film had the most BSA adsorption; hydration treatment led to migration of the biotin moieties from inner film to surface and thus resulted in less BSA adsorption; blocking layer on the polymer surface saturated the active sites for physical and chemical adsorptions on the surface and thus weakened the BSA adsorption. Adsorption of SAv displayed similar polymer-surface-status dependence, i.e., more adsorption on vacuum-dried surface, less adsorption on hydrated surface and the least adsorption on blocked surface. Compared with BSA, SAv showed more enhanced adsorptions on P(LA-co-DHC/biotin) surface because of the specific interaction of biotin moieties in the polymer with SAv molecules, especially on the blocked surface. The above semi-quantified results further indicate that the FT-SPR system is suitable for investigating interactions between polymer surface and bio-molecules.
傅里叶变换表面等离子体共振（FT-SPR）被用于通过监测链霉亲和素（SAv）和牛血清白蛋白（BSA）在聚合物膜上的吸附，来研究蛋白质与生物素化聚合物膜之间的特异性和非特异性相互作用。生物素化聚合物，聚（丙交酯-共-2,2-二羟甲基-丙碳酸酯-接枝-生物素）[P（LA-co-DHC/biotin）]，是通过丙交酯和带有 OH 的环状碳酸酯单体的开环共聚反应制备的，随后对 OH 基团进行生物素化。共聚物被涂覆到 FT-SPR 芯片上并进行真空干燥、70°C 水合处理和分别用封闭剂处理，以实现不同的表面状态。FT-SPR 结果表明，真空干燥的膜具有最多的 BSA 吸附；水合处理导致生物素部分从内部膜迁移到表面，从而导致较少的 BSA 吸附；聚合物表面上的阻挡层饱和了表面物理和化学吸附的活性位点，从而减弱了 BSA 的吸附。SAv 的吸附显示出类似的聚合物表面状态依赖性，即在真空干燥表面上吸附较多，在水合表面上吸附较少，在被阻挡的表面上吸附最少。与 BSA 相比，SAv 在 P（LA-co-DHC/biotin）表面上表现出更强的吸附，这是因为聚合物中的生物素部分与 SAv 分子之间存在特异性相互作用，特别是在被阻挡的表面上。上述半定量结果进一步表明，FT-SPR 系统适用于研究聚合物表面与生物分子之间的相互作用。
