School of Chemical Engineering and Advanced Materials, Merz Court, Newcastle University, Newcastle upon Tyne, Tyne and Wear, UK.
Bioresour Technol. 2011 Feb;102(3):2758-66. doi: 10.1016/j.biortech.2010.10.147. Epub 2010 Nov 20.
The influence of external load on the composition of the anodic biofilm microbial community and biomass yield was investigated in a microbial fuel cell fed with glucose and domestic wastewater was used as source of electrogens. Denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR) amplified 16S rRNA gene fragments revealed distinct differences in anodic bacterial communities formed at the anode of each MFC operated under a different external load. These results implied that in an MFC, electrogenic bacteria were enriched under higher current densities, i.e., low external load, and were able to sustain better current and effluent quality. The influence of the external resistance applied to the MFCs during formation of the bacterial communities from sewage wastewater was shown to have no significant effect on power performance of the MFCs nor to have a significant influence on their anodic activity with both glucose and brewery wastewater as fuel. As expected, current generation, COD removal and the biomass yield were all directly influenced by the external load. Significantly, when operated under lower external load, the biomass yield in the MFC was less than that in conventional anaerobic digestion (i.e., control).
研究了在外加负载对阳极生物膜微生物群落组成和生物量产量的影响，使用葡萄糖作为燃料的微生物燃料电池和以生活污水作为产电菌源。通过聚合酶链反应（PCR）扩增的 16S rRNA 基因片段的变性梯度凝胶电泳（DGGE）显示，在不同外加负载下运行的每个微生物燃料电池阳极上形成的阳极细菌群落存在明显差异。这些结果表明，在微生物燃料电池中，在更高的电流密度（即低外加负载）下，产电菌得到了富集，并且能够更好地维持电流和出水质量。从污水中形成细菌群落时施加到微生物燃料电池的外部电阻的影响表明，它对微生物燃料电池的功率性能没有显著影响，也没有对以葡萄糖和啤酒废水为燃料的阳极活性产生显著影响。正如预期的那样，电流生成、COD 去除和生物量产量都直接受到外加负载的影响。值得注意的是，当在较低的外加负载下运行时，微生物燃料电池中的生物量产量低于传统的厌氧消化（即对照）。
