Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhejiang Province, Zhoushan 316000, PR China.
Dev Comp Immunol. 2011 Apr;35(4):483-9. doi: 10.1016/j.dci.2010.12.003. Epub 2010 Dec 13.
Major histocompatibility complex (MHC) has a central role in the adaptive immune system by presenting foreign peptide to the T-cell receptor. MHC gene family contains two main subgroups of immunologically active molecules. In order to study the molecular function and genomic characteristic of class I gene in teleost, the full lengths of MHC class Iα cDNA and genomic sequence were cloned from miiuy croaker (Miichthys miiuy). Seven exons and six introns were identified in miiuy croaker class Iα gene. This genomic structural feature of miiuy croaker is similar to that present in some fishes such as Japanese flounder and Atlantic salmon, but different from that present in some other fishes such as half-smooth tongue sole and channel catfish. The deduced amino acid sequence of class Iα gene had 25.9-54.1% identity with those of mammal and teleost. Real-time quantitative RT-PCR demonstrated that the MHC class Iα gene was ubiquitously expressed in 10 normal tissues; expression levels of MHC Iα gene were found first upregulated and then downregulated throughout the pathogenic bacteria infection process in spleen and kidney.
主要组织相容性复合体 (MHC) 通过向 T 细胞受体呈递外来肽，在适应性免疫系统中发挥核心作用。MHC 基因家族包含两类具有免疫活性的分子。为了研究硬骨鱼类 MHC I 类基因的分子功能和基因组特征，从米氏绒螯蟹 (Miichthys miiuy) 中克隆了 MHC Iα cDNA 的全长和基因组序列。在米氏绒螯蟹的 MHC Iα 基因中鉴定出 7 个外显子和 6 个内含子。这种米氏绒螯蟹的基因组结构特征类似于日本牙鲆和大西洋鲑等鱼类，但与半滑舌鳎和斑点叉尾鮰等其他鱼类不同。MHC Iα 基因的推导氨基酸序列与哺乳动物和硬骨鱼类的氨基酸序列具有 25.9-54.1%的同源性。实时定量 RT-PCR 表明，MHC Iα 基因在 10 种正常组织中广泛表达；在脾脏和肾脏的整个致病菌感染过程中，发现 MHC Iα 基因的表达水平先上调后下调。
