Department of Biomedical Sciences and Technologies, University of Udine, Udine, Italy.
Mitochondrion. 2011 Mar;11(2):315-26. doi: 10.1016/j.mito.2010.12.007. Epub 2010 Dec 13.
H9c2 undergoing cardiac differentiation induced by all-trans-retinoic acid were investigated for mitochondria structural features together with the implied functional changes, as a model for the study of mitochondrial development in cardiogenic progenitor cells. As the expression of cardiac markers became detectable, mitochondrial mass increased and mitochondrial morphology and ultrastructure changed. Reticular network organization developed and more bulky mitochondria with greater numbers of closely packed cristae and more electron-dense matrix were detected. Increased expression of PGC-1α proved the occurrence of mitochondrial biogenesis. Improvements in mitochondrial energetic competence were also documented, linked to better assembly between F(0) and F(1) sectors of the F(0)F(1)ATPsynthase enzyme complex.
用全反式视黄酸诱导 H9c2 细胞向心肌细胞分化，研究其线粒体结构特征及潜在的功能变化，作为研究成心机细胞中线粒体发育的模型。随着心肌标志物的表达变得可检测，线粒体质量增加，线粒体形态和超微结构发生变化。网状网络组织发育，检测到具有更多紧密堆积的嵴和更密集的电子致密基质的更大体积的线粒体。PGC-1α 的表达增加证明了线粒体生物发生的发生。还记录了线粒体能量能力的改善，这与 F(0)F(1)ATP 合酶酶复合物的 F(0)和 F(1) 部分之间更好的组装有关。
