Division of Biological Sciences, Chonbuk National University, Chonju, Chonbuk 561-756, Republic of Korea.
Mycologia. 2004 Jan-Feb;96(1):52-6.
The presence of a mannoprotein, MnpAp, in the hyphal cell wall of Aspergillus nidulans was examined by immunogold electron microscopy using a mnpA-null mutant as a negative control. The hyphal cell wall of wild type consisted of two layers-an electron-dense smooth outer layer and an electron-translucent inner layer-while the hyphal cell wall of the mnpA-null mutant had an electron-dense irregular outer layer together with the electron-translucent inner layer. In wild type, MnpAp was present throughout the electron-translucent layer of the hyphal cell wall but was absent from the conidial cell wall. In the mnpA-null mutant, MnpAp was absent from the cell walls of both cell types. These results indicate that MnpAp is present in the hyphal cell wall and that it influences cell wall surface structure.
通过使用 mnpA 缺失突变体作为阴性对照的免疫金电子显微镜检查，研究了 Aspergillus nidulans 丝状细胞细胞壁中甘露糖蛋白 MnpAp 的存在。野生型丝状细胞细胞壁由两层组成——电子致密的光滑外层和电子透明的内层，而 mnpA 缺失突变体的丝状细胞细胞壁则具有电子致密的不规则外层和电子透明的内层。在野生型中，MnpAp 存在于丝状细胞细胞壁的整个电子透明层中，但不存在于分生孢子细胞壁中。在 mnpA 缺失突变体中，MnpAp 不存在于两种细胞类型的细胞壁中。这些结果表明 MnpAp 存在于丝状细胞细胞壁中，并影响细胞壁表面结构。
