Department of Biological Sciences, University of Maine, Orono, Maine 04469-5722.
Mycologia. 2004 Jan-Feb;96(1):162-71.
Rhizophydium136 was isolated from pollen bait placed in a water culture containing garden soil from Penobscot County, Maine. It is an important isolate because its entire mitochondrial genome has been sequenced and it is the representative member of the Chytridiales in a fungal phylogeny based on mitochondrial protein sequences. Also, this isolate is included in an 18S rDNA, chytrid phylogeny. On nutrient agar, many inflated rhizoidal axes extend from the base of the zoosporangium, zoosporangia mature in 3 d and zoospores discharge through numerous, lenticular, discharge pores. Smooth-walled resting spores form in crowded cultures. Zoospores are a variation of the Rhizophydium subtype. This chytrid differs from R. sphaerotheca sensu Barr and because it cannot be placed in a described species it herein is described as Rhizophydium brooksianum sp. nov. Many of the differences between Rhizophydium brooksianum and other multipored Rhizophydium isolates were observed only in pure culture. Attributing a spherical, multipored Rhizophydium to a species that was described without developmental information from pure culture is untenable. Epitypes or neotypes for inadequately characterized species need to be selected, and cultures made available.
从缅因州彭诺斯科特县花园土壤的水培中放置的花粉诱饵中分离出 Rhizophydium136。它是一个重要的分离物，因为其整个线粒体基因组已经测序，并且它是基于线粒体蛋白序列的真菌系统发育中 Chytridiales 的代表成员。此外，该分离物包含在 18S rDNA、chytrid 系统发育中。在营养琼脂上，许多膨胀的根状轴从游动孢子囊的基部延伸出来，游动孢子囊在 3 天内成熟，通过许多透镜状的排放孔释放游动孢子。在密集的培养物中形成光滑壁的休眠孢子。游动孢子是 Rhizophydium 亚型的一种变体。这种壶菌与 Rhizophydium sphaerotheca sensu Barr 不同，由于它不能被归入已描述的物种，因此在此将其描述为 Rhizophydium brooksianum sp. nov.。在纯培养中仅观察到 Rhizophydium brooksianum 与其他多孔 Rhizophydium 分离物之间的许多差异。将具有球形、多孔的 Rhizophydium 归因于一种没有纯培养发育信息的物种是站不住脚的。需要选择足够特征描述的不充分描述物种的副模式或新正模式，并提供培养物。
