Norwegian Forest Research Institute, N-1432 Ås, Norway.
Mycologia. 2004 Nov-Dec;96(6):1352-4.
Seiridium cardinale, S. cupressi and S. unicorne represent three distinct species of fungi that cause cankers on Cupressus species and the disease collectively known as cypress canker. These fungi cannot be distinguished reliably from each other using morphological characters or ribosomal DNA sequence data. Here we describe a RFLP assay based on digesting β-tubulin amplicons with a single endonuclease, HaeIII, which easily can be used to distinguish among these three species. This RFLP assay provides an inexpensive and simple means of identifying Seiridium species, which include some of the most serious threats to trees in Cupressaceae.
栗疫病菌、柏肤小穴壳菌和栓皮长喙壳菌代表了三种不同的真菌物种，它们会引起柏科植物的溃疡病，通常被称为柏溃疡病。这些真菌不能仅通过形态特征或核糖体 DNA 序列数据可靠地区分彼此。在这里，我们描述了一种基于用单个内切酶 HaeIII 消化β-微管蛋白扩增子的 RFLP 分析方法，该方法可以轻松地区分这三种真菌。该 RFLP 分析方法为鉴定包括对柏科树木最严重威胁的一些种的 Seiridium 物种提供了一种廉价且简单的手段。
