Jhanwar S C, de Sostoa A, Doucette L A, Posnett D N
Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Cytogenet Cell Genet. 1990;53(2-3):155-60. doi: 10.1159/000132918.
We report here a cytogenetic and molecular analysis of two cases of T-cell leukemia with t(14;14) (q11.2;q32). Through in situ hybridization and Southern blotting, using radioactively labeled immunoglobulin heavy chain (IGH) and alpha T-cell receptor (TCRA) gene probes, we found in both tumors that the loci of both IGH and TCRA were rearranged. Molecular analysis of the t(14;14) clearly demonstrated that in some tumors rearrangements of the IGH and TCRA genes are associated with interchromosomal exchanges that result in specific chromosome translocations that confer a proliferative advantage and predisposition to leukemic transformation. The implication of these rearrangements for normal and neoplastic T-cell development is discussed.
我们在此报告两例伴有t(14;14)(q11.2;q32)的T细胞白血病的细胞遗传学和分子分析。通过原位杂交和Southern印迹法，使用放射性标记的免疫球蛋白重链(IGH)和α T细胞受体(TCRA)基因探针，我们在两个肿瘤中均发现IGH和TCRA基因座发生了重排。对t(14;14)的分子分析清楚地表明，在某些肿瘤中，IGH和TCRA基因的重排与染色体间交换有关，这些交换导致特定的染色体易位，赋予增殖优势并易患白血病转化。讨论了这些重排对正常和肿瘤性T细胞发育的影响。
