Department of Genetics & Biochemistry, Clemson University, Clemson, SC, USA.
Plant Signal Behav. 2010 Dec;5(12):1657-9. doi: 10.4161/psb.5.12.14000. Epub 2010 Dec 1.
A novel autoregulation of nodulation locus in Medicago truncatula, lss, silences the SUNN gene thorough a cis-acting mechanism. Microarray analysis was performed on the Affymetrix Gene Chip® Medicago Genome Array with cDNA isolated from seven-day-old seedlings of wild type, sunn-1 and lss plants. The results suggest that in lss plants expression of only a few dozen genes differs significantly from wild type while in sunn-1 plants expression of several hundred genes represented by over 800 probe sets is altered. These results suggest that the kinase domain modification caused by the sunn-1 mutation alters the receptor's influence on gene expression and that these differences are present even in the absence of nodulation.
在蒺藜苜蓿中，一个新的结瘤调控位点 lss 通过顺式作用机制沉默 SUNN 基因。利用 Affymetrix Gene Chip® Medicago 基因组芯片从野生型、sunn-1 和 lss 植株的 7 天龄幼苗中分离的 cDNA 进行微阵列分析。结果表明，在 lss 植株中，只有几十种基因的表达与野生型显著不同，而在 sunn-1 植株中，有几百种基因（由 800 多个探针集表示）的表达发生了改变。这些结果表明，sunn-1 突变引起的激酶结构域修饰改变了受体对基因表达的影响，即使在没有结瘤的情况下，这些差异也存在。
