Subharat S, Wilson P R, Heuer C, Collins-Emerson J M
Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North 4442, New Zealand.
N Z Vet J. 2010 Dec;58(6):281-5. doi: 10.1080/00480169.2010.69755.
To find evidence for localisation in the uterus, and fetal infection, of Leptospira spp. in farmed deer in the lower North Island of New Zealand during and shortly after the breeding season.
Between February and July 2008, 116 blood samples, 120 kidneys, 120 uteri and 27 fetuses were collected from 120 mixed-age hinds from lines from nine farms, at a deer slaughter premises. Serum samples were tested for antibodies against Leptospira borgpetersenii serovar Hardjo-bovis and Leptospira interrogans serovar Pomona, using the microscopic agglutination test (MAT). For both serovars, a titre of ≥1:48 was considered positive. Samples from kidneys, uteri and fetal tissue were subjected to bacterial culture, using Ellinghausen-McCullough-Johnson-Harris (EMJH) medium, and real-time PCR, using DNA gyrase subunit B gene primers.
Thirty-four of 116 (29.3%) serum samples were positive for serovar Hardjo-bovis, and 13 (11.2%) for serovar Pomona. Seven of 120 kidneys were positive for serovar Hardjo-bovis by culture, and five of these, but no others, were positive by real-time PCR. Of 120 uteri, none was culture- or PCR-positive. None of 27 fetal samples was culture-positive but one was positive by real-time PCR. The dam of the PCR-positive fetus was culture-negative from the kidney, but had an MAT titre of 1:192 for Hardjo-bovis.
Attempts to isolate Leptospira spp. from the genital tracts and early fetuses of farmed deer were unsuccessful. However, molecular evidence suggested fetal infection in one case. This finding justifies further study of the role of leptospires in the genital tract and fetus and its association with reproductive loss in farmed deer.
寻找新西兰北岛下部养殖鹿在繁殖季节期间及之后不久钩端螺旋体在子宫内的定位及胎儿感染的证据。
2008年2月至7月间，在一家鹿屠宰场从9个农场的120头不同年龄的母鹿身上采集了116份血液样本、120个肾脏、120个子宫和27个胎儿样本。使用显微镜凝集试验（MAT）检测血清样本中针对波摩那钩端螺旋体血清型波摩那和波氏钩端螺旋体血清型哈德乔-波维斯的抗体。对于这两种血清型，滴度≥1:48被视为阳性。肾脏、子宫和胎儿组织样本使用埃林豪森-麦卡洛-约翰逊-哈里斯（EMJH）培养基进行细菌培养，并使用DNA促旋酶亚基B基因引物进行实时PCR。
116份血清样本中有34份（29.3%）波氏钩端螺旋体血清型哈德乔-波维斯呈阳性，13份（11.2%）波摩那钩端螺旋体血清型波摩那呈阳性。120个肾脏中有7个通过培养波氏钩端螺旋体血清型哈德乔-波维斯呈阳性，其中5个通过实时PCR呈阳性，其他肾脏均为阴性。120个子宫中，培养或PCR均未呈阳性。27个胎儿样本中培养均未呈阳性，但1个通过实时PCR呈阳性。PCR阳性胎儿的母体肾脏培养为阴性，但波氏钩端螺旋体血清型哈德乔-波维斯的MAT滴度为1:192。
从养殖鹿的生殖道和早期胎儿中分离钩端螺旋体未获成功。然而，分子证据表明有一例胎儿感染。这一发现证明有必要进一步研究钩端螺旋体在生殖道和胎儿中的作用及其与养殖鹿繁殖损失的关联。
