Barth Ortrud M, Freitas Alex S, Oliveira Erika S, Silva Rosana A, Maester Fernanda M, Andrella Raquel R S, Cardozo Gina M B Q
Laboratório de Palinologia, Departamento de Botânica, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Centro de Ciências da Saúde, Bloco A, Sala, Rio de Janeiro, RJ, Brasil.
An Acad Bras Cienc. 2010 Dec;82(4):893-902. doi: 10.1590/s0001-37652010000400011.
High quality of bee pollen for commercial purpose is required. In order to attend the consumer with the best identification of the botanical and floral origin of the product, 25 bee pollen batches were investigated using two techniques of pollen grain preparation. The first started to identify pollen loads of different colors in two grams of each well mixed batch, and the second to identify pollen grains in a pool made of all the pollen loads comprised in two grams. The best result was obtained by this last technique, when a pollen grain suspension was dropped on a microscope slide and circa 500 pollen grains were counted per sample. This analysis resulted in the recognition of monofloral and bifloral pollen batches, while the use of the first technique resulted in all samples receiving a heterofloral diagnosis.
需要高质量的用于商业目的的蜂花粉。为了让消费者能够最好地识别产品的植物和花卉来源，使用两种花粉粒制备技术对25批蜂花粉进行了研究。第一种方法是在每两克充分混合的批次中识别不同颜色的花粉负载，第二种方法是在由两克中包含的所有花粉负载组成的混合样本中识别花粉粒。当将花粉粒悬浮液滴在显微镜载玻片上，每个样本计数约500个花粉粒时，最后一种技术获得了最佳结果。该分析结果识别出了单花和双花花粉批次，而使用第一种技术时，所有样本都被诊断为多花花粉。
