使用不同成熟培养液对绵羊卵母细胞进行体外成熟培养：人绝经期血清的影响。
In vitro maturation of ovine oocytes using different maturation media: effect of human menopausal serum.
机构信息
Department of Animal Science, Razi University, Kermanshah, Iran.
出版信息
J Assist Reprod Genet. 2011 Jun;28(6):531-7. doi: 10.1007/s10815-010-9523-3. Epub 2010 Dec 9.
OBJECTIVE
To compare the effect of human menopausal serum with estrous sheep serum, estrous goat serum, ovine follicular fluid and bovine follicular fluid on in vitro maturation, in vitro fertilization and embryo development of sheep oocytes METHOD (S): Oocytes were treated in culture with different sera and follicular fluids supplemented media to examine effects on embryo development.
RESULTS
Basic culture medium supplemented with human menopausal serum, estrous sheep serum and estrous goat serum supported better rates of in vitro maturation, in vitro fertilization and embryo development. Ovine follicular fluid and bovine follicular fluid supplementations supported similar rates of In vitro maturation, In vitro fertilization and embryo development which were lower than those supported by human menopausal serum, estrous sheep serum, estrous goat serum and control medium.
CONCLUSION
Human menopausal serum, estrous sheep serum, and estrous goat serum resulted in higher maturation, fertilization and embryo development than ovine follicular fluid, bovine follicular fluid and control media.
目的
比较人绝经期血清与发情期绵羊血清、发情期山羊血清、绵羊卵泡液和牛卵泡液对绵羊卵母细胞体外成熟、体外受精和胚胎发育的影响。
方法
用不同的血清和卵泡液补充培养基处理卵母细胞，以观察对胚胎发育的影响。
结果
基础培养基中添加人绝经期血清、发情期绵羊血清和发情期山羊血清，体外成熟、体外受精和胚胎发育的支持率较高。绵羊卵泡液和牛卵泡液的补充支持的体外成熟、体外受精和胚胎发育率与对照组相似，均低于人绝经期血清、发情期绵羊血清、发情期山羊血清和对照组。
结论
人绝经期血清、发情期绵羊血清和发情期山羊血清比绵羊卵泡液、牛卵泡液和对照组能获得更高的成熟率、受精率和胚胎发育率。
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