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In vitro production of bovine embryos in medium supplemented with a serum replacer: effects on blastocyst development, cryotolerance and survival to term.在添加血清替代物的培养基中体外生产牛胚胎：对囊胚发育、耐冻性和足月存活率的影响。
Theriogenology. 2007 Dec;68(9):1316-25. doi: 10.1016/j.theriogenology.2007.08.034. Epub 2007 Oct 10.
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Developmental potential of bovine oocytes cultured in different maturation and culture conditions.在不同成熟和培养条件下培养的牛卵母细胞的发育潜能。
Anim Reprod Sci. 2007 Oct;101(3-4):225-40. doi: 10.1016/j.anireprosci.2006.09.016. Epub 2006 Oct 18.
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Comparison of various maturation treatments on in vitro maturation of goat oocytes and their early embryonic development and cell numbers.不同成熟处理对山羊卵母细胞体外成熟、早期胚胎发育及细胞数量的比较
Theriogenology. 1996 Oct 15;46(6):971-82. doi: 10.1016/s0093-691x(96)00261-0.
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Effect of oocyte-sperm co-incubation on acrosome reaction in the goat.
Theriogenology. 1996 Jul 15;46(2):321-30. doi: 10.1016/0093-691x(96)00188-4.
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The effects of follicular fluid on in vitro maturation, oocyte fertilization and the development of bovine embryos.
Theriogenology. 1996 Mar;45(4):787-99. doi: 10.1016/0093-691x(96)00008-8.
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Development of in vitro matured and fertilized bovine embryos, cultured from days 1-5 post insemination in either Menezo-B2 medium or in HECM-6 medium.
Theriogenology. 1995 Nov;44(7):935-45. doi: 10.1016/0093-691x(95)00281-c.
7
Factors affecting bovine embryo development in synthetic oviduct fluid following oocyte maturation and fertilization in vitro.体外卵母细胞成熟和受精后，合成输卵管液中影响牛胚胎发育的因素。
Theriogenology. 1995 Apr 15;43(6):1115-28. doi: 10.1016/0093-691x(95)00075-j.
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Ultrastructural studies of developing goat oocytes in vitro.
Theriogenology. 1994 Nov 1;42(6):1003-16. doi: 10.1016/0093-691x(94)90122-y.
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Term development of caprine embryos derived from immature oocytes in vitro.
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Influence of epidermal growth factor and insulin-like growth factor 1 on nuclear maturation and fertilization of buffalo cumulus oocyte complexes in serum free media and their subsequent development in vitro.表皮生长因子和胰岛素样生长因子1对水牛卵丘卵母细胞复合体在无血清培养基中核成熟和受精的影响及其随后的体外发育
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使用不同成熟培养液对绵羊卵母细胞进行体外成熟培养：人绝经期血清的影响。

In vitro maturation of ovine oocytes using different maturation media: effect of human menopausal serum.

机构信息

Department of Animal Science, Razi University, Kermanshah, Iran.

出版信息

J Assist Reprod Genet. 2011 Jun;28(6):531-7. doi: 10.1007/s10815-010-9523-3. Epub 2010 Dec 9.

DOI:10.1007/s10815-010-9523-3
PMID:21152965
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3158248/
Abstract

OBJECTIVE

To compare the effect of human menopausal serum with estrous sheep serum, estrous goat serum, ovine follicular fluid and bovine follicular fluid on in vitro maturation, in vitro fertilization and embryo development of sheep oocytes METHOD (S): Oocytes were treated in culture with different sera and follicular fluids supplemented media to examine effects on embryo development.

RESULTS

Basic culture medium supplemented with human menopausal serum, estrous sheep serum and estrous goat serum supported better rates of in vitro maturation, in vitro fertilization and embryo development. Ovine follicular fluid and bovine follicular fluid supplementations supported similar rates of In vitro maturation, In vitro fertilization and embryo development which were lower than those supported by human menopausal serum, estrous sheep serum, estrous goat serum and control medium.

CONCLUSION

Human menopausal serum, estrous sheep serum, and estrous goat serum resulted in higher maturation, fertilization and embryo development than ovine follicular fluid, bovine follicular fluid and control media.

摘要

目的

比较人绝经期血清与发情期绵羊血清、发情期山羊血清、绵羊卵泡液和牛卵泡液对绵羊卵母细胞体外成熟、体外受精和胚胎发育的影响。

方法

用不同的血清和卵泡液补充培养基处理卵母细胞，以观察对胚胎发育的影响。

结果

基础培养基中添加人绝经期血清、发情期绵羊血清和发情期山羊血清，体外成熟、体外受精和胚胎发育的支持率较高。绵羊卵泡液和牛卵泡液的补充支持的体外成熟、体外受精和胚胎发育率与对照组相似，均低于人绝经期血清、发情期绵羊血清、发情期山羊血清和对照组。

结论

人绝经期血清、发情期绵羊血清和发情期山羊血清比绵羊卵泡液、牛卵泡液和对照组能获得更高的成熟率、受精率和胚胎发育率。