Kontogeorgos G, Kovacs K
Department of Pathology, General Hospital of Athens, 154 Messogion Avenue, 115 27, Athens, Greece,
Endocrine. 1996 Dec;5(3):235-40. doi: 10.1007/BF02739055.
Fluorescencein situ hybridization (FISH) is currently recognized as a reliable, sensitive, and reproducible technique for identification of copy number and structure of chromosomes providing information on the single-cell level. The technique permits cytogenetic investigation of metaphase spreads and interphase nuclei. Several protocols have been used for preparations from fresh samples or archival material. Alphoid or telomeric DNA probes can detect specific chromosomes, and cosmid probes can localize single copies of the segment of interest. FISH is a powerful tool in understanding physiologic mechanisms, in generating high-resolution physical genetic maps, and in resolving problems of the pathogenesis of several diseases. FISH may shed light on the cytogenetic background and chromosomal alterations in the field of endocrinology, resulting in a better understanding of functional activities and various endocrine disorders.
荧光原位杂交（FISH）目前被认为是一种可靠、灵敏且可重复的技术，用于识别染色体的拷贝数和结构，可在单细胞水平上提供信息。该技术允许对中期染色体 spreads 和间期核进行细胞遗传学研究。已经使用了几种方案来从新鲜样本或存档材料中进行制备。α卫星或端粒 DNA 探针可以检测特定染色体，黏粒探针可以定位感兴趣片段的单拷贝。FISH 是理解生理机制、生成高分辨率物理遗传图谱以及解决几种疾病发病机制问题的有力工具。FISH 可能有助于阐明内分泌学领域的细胞遗传学背景和染色体改变，从而更好地理解功能活动和各种内分泌疾病。
