Li R, Gao W Q, Mather J P
Cell Biology, Genetech, 460 Point San Bruno Blvd., 94080, South San Francisco, CA.
Endocrine. 1996 Oct;5(2):205-17. doi: 10.1007/BF02738708.
The proliferation and differentiation of neural precursor cells is largely controlled by environmental factors. By providing the factors that favor the proliferation or suppress the differentiation of this cell type, we isolated and expanded an early neuroepithelial pre-differentiated cell type from E9 rat neural plate in serum-free medium. This has led to the establishment of a neural epithelial precursor (NEP) cell line. The NEP cell's properties are substantially different from those of cell lines previously derived from neural tissue at later stages of development. Initial selection and survival of this cell type requires a factor secreted by an embryonic Schwann (nrESC) cell line. Continued passage of these cells requires cell-cell contact for both survival and growth. Neural cell differentiation can be induced in this nestin positive precursor cell line by bFGF and forskolin. General neuronal markers, as well as cortical neuron-specific protein kinase C isozyme, and accumulation of glutamate and aspartate were induced in most cells. Choline acetyl-transferase was also induced in a small number of cells. When implanted into neonatal rat brain, the NEP cell line gave rise to several distinct neuronal and glial phenotypes in different regions of the brain including cerebellar cortex and hippocampus.
神经前体细胞的增殖和分化很大程度上受环境因素控制。通过提供有利于这种细胞类型增殖或抑制其分化的因子，我们在无血清培养基中从E9大鼠神经板分离并扩增了一种早期神经上皮预分化细胞类型。这导致了一种神经上皮前体细胞（NEP）系的建立。NEP细胞的特性与先前在发育后期从神经组织衍生的细胞系有很大不同。这种细胞类型的初始选择和存活需要胚胎雪旺氏细胞系（nrESC）分泌的一种因子。这些细胞的持续传代需要细胞间接触以实现存活和生长。bFGF和福斯高林可诱导这种巢蛋白阳性前体细胞系发生神经细胞分化。大多数细胞中诱导出了一般神经元标志物、皮质神经元特异性蛋白激酶C同工酶以及谷氨酸和天冬氨酸的积累。少数细胞中还诱导出了胆碱乙酰转移酶。当植入新生大鼠脑内时，NEP细胞系在包括小脑皮质和海马体在内的脑的不同区域产生了几种不同的神经元和神经胶质细胞表型。
