Rajagopala Raja C A, Spicer L J, Stewart R E
Department of Animal Science, Oklahoma State University, 74078, Stillwater, OK, USA.
Endocrine. 1995 Dec;3(12):899-905. doi: 10.1007/BF02738895.
The effect of recombinant bovine interleukin-2 (IL-2) on steroidogenesis and numbers of bovine ovarian granulosa and thecal cells has been studied. Granulosa cells have been examined from both small (surface diameter ≤5 mm) and large (≥8 mm) follicles, whereas thecal cells from only large follicles were utilized. Estradiol and progesterone production per cell by granulosa cells from large follicles was 2- to 3-times greater than those from small follicles. Increasing doses of IL-2 significantly attenuated FSH-induced estradiol production by cells from small follicles but not large follicles. In general, progesterone production per cell by granulosa cells was almost double that of thecal cells. Moreover, IL-2 significantly attenuated FSH-induced progesterone production by granulosa cells from small and large follicles but had no effect on LH-induced progesterone or and-rostenedione production by thecal cells. Co-treatment of TNFα with IL-2 enhanced the responsiveness of granulosa cells to IL-2. The effect of IL-2 on the numbers of granulosa and thecal cells were studied independently under serum-free conditions and media enriched with 10% fetal calf serum. In serum-free medium containing insulin, IL-2 dosage significantly increased numbers of granulosa cells from large follicles, whereas IL-2 had no effect on numbers of granulosa cells from small follicles or thecal cells from large follicles. When cells were grown in medium enriched with serum, increasing doses of IL-2 significantly inhibited numbers without affecting viability of granulosa cells from small follicles, but had no effect on numbers of thecal cells. Thus, it appears that granulosa cells are more sensitive to IL-2 than are thecal cells.
研究了重组牛白细胞介素-2（IL-2）对牛类固醇生成以及牛卵巢颗粒细胞和卵泡膜细胞数量的影响。对小卵泡（表面直径≤5mm）和大卵泡（≥8mm）的颗粒细胞均进行了检测，而仅使用了大卵泡的卵泡膜细胞。大卵泡颗粒细胞每细胞产生的雌二醇和孕酮量比小卵泡颗粒细胞高2至3倍。增加剂量的IL-2显著减弱了促卵泡激素（FSH）诱导的小卵泡细胞而非大卵泡细胞的雌二醇生成。一般而言，颗粒细胞每细胞产生的孕酮量几乎是卵泡膜细胞的两倍。此外，IL-2显著减弱了FSH诱导的小卵泡和大卵泡颗粒细胞的孕酮生成，但对促黄体生成素（LH）诱导的卵泡膜细胞孕酮或雄烯二酮生成没有影响。肿瘤坏死因子α（TNFα）与IL-2共同处理增强了颗粒细胞对IL-2的反应性。在无血清条件和富含10%胎牛血清的培养基中分别研究了IL-2对颗粒细胞和卵泡膜细胞数量的影响。在含有胰岛素的无血清培养基中，IL-2剂量显著增加了大卵泡颗粒细胞的数量，而IL-2对小卵泡颗粒细胞或大卵泡卵泡膜细胞的数量没有影响。当细胞在富含血清的培养基中生长时，增加剂量的IL-2显著抑制了小卵泡颗粒细胞的数量，但不影响其活力，而对卵泡膜细胞数量没有影响。因此，似乎颗粒细胞比卵泡膜细胞对IL-2更敏感。
